• Featured Product
  • KD/KO验证

UAP1 Monoclonal antibody

UAP1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67545-1-Ig

产品说明书

CloneNo. 2C7B2

宿主/亚型

Mouse / IgG1

种属反应性

Human and More (1)

应用

WB, IHC, IF/ICC, ELISA and More (1)

AgX, AGX 2, AGX1, Antigen X, SPAG2, UAP1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells, A549 cells, NCI-H1299 cells, LNCaP cells, HepG2 cells, THP-1 cells, HEK-293 cells, DU 145 cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-3 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67545-1-Ig targets UAP1 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, CoIP
经测试反应性 Human
文献引用反应性human
免疫原 UAP1 fusion protein Ag9513 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 UDP-N-acteylglucosamine pyrophosphorylase 1
别名 AgX, AGX 2, AGX1, Antigen X, SPAG2, UAP1
计算分子量 505 aa, 57 kDa
观测分子量 57-59 kDa
GenBank蛋白编号BC009377
基因名称 UAP1
Gene ID (NCBI) 6675
RRIDAB_2882762
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ16222
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

UAP1 can convert UTP and GlcNAc-1-P into UDP-GlcNAc, it can also convert UTP and GalNAc-1-P into UDP-GalNAc. There are three isoforms of UAP1, among which isoform  AGX1  has 2 to 3 times higher activity towards GalNAc-1-P, while isoform  AGX2  has 8 times more activity towards GlcNAc-1-P. It has been reported that UAP1 is specific and highly expressed in prostate cancer. UAP1 can protect cells against inhibitors of N-linked glycosylation. UAP1 may play an important role in maintaining INS responsiveness. 67545-1-Ig detects 57 and 59 kDa isoforms in SDS-PAGE.(PMID:25241896)

实验方案

Product Specific Protocols
WB protocol for UAP1 antibody 67545-1-IgDownload protocol
IHC protocol for UAP1 antibody 67545-1-IgDownload protocol
IF protocol for UAP1 antibody 67545-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,CoIP

Cell Res

Mannose antagonizes GSDME-mediated pyroptosis through AMPK activated by metabolite GlcNAc-6P

Authors - Yuan-Li Ai
humanWB

Mol Cell

Metabolic enzyme UAP1 mediates IRF3 pyrophosphorylation to facilitate innate immune response

Authors - Shuai Yang
  • KD Validated
humanIHC,WB

Front Immunol

The hexosamine biosynthesis pathway-related gene signature correlates with immune infiltration and predicts prognosis of patients with osteosarcoma

Authors - Zexin Su
Loading...