验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, A549 cells, NCI-H1299 cells, LNCaP cells, HepG2 cells, THP-1 cells, HEK-293 cells, DU 145 cells, Jurkat cells, K-562 cells |
Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | PC-3 cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
67545-1-Ig targets UAP1 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with Human samples.
经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
文献引用应用 | WB, IHC, CoIP |
经测试反应性 | Human |
文献引用反应性 | human |
免疫原 | UAP1 fusion protein Ag9513 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | UDP-N-acteylglucosamine pyrophosphorylase 1 |
别名 | AgX, AGX 2, AGX1, Antigen X, SPAG2, UAP1 |
计算分子量 | 505 aa, 57 kDa |
观测分子量 | 57-59 kDa |
GenBank蛋白编号 | BC009377 |
基因名称 | UAP1 |
Gene ID (NCBI) | 6675 |
RRID | AB_2882762 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | Q16222 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
UAP1 can convert UTP and GlcNAc-1-P into UDP-GlcNAc, it can also convert UTP and GalNAc-1-P into UDP-GalNAc. There are three isoforms of UAP1, among which isoform AGX1 has 2 to 3 times higher activity towards GalNAc-1-P, while isoform AGX2 has 8 times more activity towards GlcNAc-1-P. It has been reported that UAP1 is specific and highly expressed in prostate cancer. UAP1 can protect cells against inhibitors of N-linked glycosylation. UAP1 may play an important role in maintaining INS responsiveness. 67545-1-Ig detects 57 and 59 kDa isoforms in SDS-PAGE.(PMID:25241896)
实验方案
Product Specific Protocols | |
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WB protocol for UAP1 antibody 67545-1-Ig | Download protocol |
IHC protocol for UAP1 antibody 67545-1-Ig | Download protocol |
IF protocol for UAP1 antibody 67545-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Res Mannose antagonizes GSDME-mediated pyroptosis through AMPK activated by metabolite GlcNAc-6P | ||
Mol Cell Metabolic enzyme UAP1 mediates IRF3 pyrophosphorylation to facilitate innate immune response
| ||
Front Immunol The hexosamine biosynthesis pathway-related gene signature correlates with immune infiltration and predicts prognosis of patients with osteosarcoma |