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UACA Polyclonal antibody

UACA Polyclonal Antibody for IF/ICC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

IF/ICC, IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 25654-1-AP

Print datasheet

Synonyms

KIAA1561, NUCLING, UACA

验证数据展示

  • IHC staining of human colon using 25654-1-AP

    IHC staining of human colon using 25654-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25654-1-AP (UACA antibody) at dilution of 1:200 (under 40x lens).

  • WB analysis of HeLa using 25654-1-AP

    WB analysis of HeLa using 25654-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25654-1-AP (UACA Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of A375 using 25654-1-AP

    IF Staining of A375 using 25654-1-AP

    Immunofluorescent analysis of A375 cells using 25654-1-AP (UACA antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, A375 cells, HEK-293 cells, Neuro-2a cells, Y79 cells
Positive IHC detected inhuman colon tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA375 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 3 publications below

产品信息

The immunogen of 25654-1-AP is UACA Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IHC, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman, canine
Immunogen UACA fusion protein Ag22438 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name uveal autoantigen with coiled-coil domains and ankyrin repeats
Synonyms KIAA1561, NUCLING, UACA
Calculated Molecular Weight 1416 aa, 163 kDa
Observed Molecular Weight 163 kDa
GenBank Accession NumberBC113407
Gene Symbol UACA
Gene ID (NCBI) 55075
RRIDAB_2880179
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BZF9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for UACA antibody 25654-1-APDownload protocol
IHC protocol for UACA antibody 25654-1-APDownload protocol
IF protocol for UACA antibody 25654-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Aging (Albany NY)

Transcriptome and proteome analysis of the antitumor activity of maslinic acid against pancreatic cancer cells

Authors - Hewei Zhang
View Article
humanWB

J Oncol

Maslinic Acid Inhibits the Growth of Malignant Gliomas by Inducing Apoptosis via MAPK Signaling.

Authors - Yongqiang Wang
View Article
canineWB

Cell Rep

Rab39 and its effector UACA regulate basolateral exosome release from polarized epithelial cells.

Authors - Takahide Matsui
View Article