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验证数据展示

  • WB analysis using 31291-1-AP

    WB analysis using 31291-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31291-1-AP (Tyrosinase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human skin cancer using 31291-1-AP

    IHC staining of human skin cancer using 31291-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 31291-1-AP (Tyrosinase antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tyrosinase Polyclonal antibody

Tyrosinase Polyclonal Antibody for WB, IHC, ELISA
Cat No. 31291-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

TYR, OCAIA, OCA1A, Monophenol monooxygenase, LB24-AB

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA375 cells, mouse liver tissue, mouse skin tissue, rat skin tissue
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31291-1-AP targets Tyrosinase in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag34940

Product name: Recombinant human Tyrosinase protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 19-200 aa of BC027179

Sequence: HFPRACVSSKNLMEKECCPPWSGDRSPCGQLSGRGSCQNILLSNAPLGPQFPFTGVDDRESWPSVFYNRTCQCSGNFMGFNCGNCKFGFWGPNCTERRLLVRRNIFDLSAPEKDKFFAYLTLAKHTISSDYVIPIGTYGQMKNGSTPMFNDINIYDLFVWMHYYVSMDALLGGSEIWRDIDF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tyrosinase (oculocutaneous albinism IA)
别名 TYR, OCAIA, OCA1A, Monophenol monooxygenase, LB24-AB
计算分子量 529 aa, 60 kDa
观测分子量 70 kDa
GenBank蛋白编号BC027179
基因名称 TYR
Gene ID (NCBI) 7299
RRIDAB_3669932
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP14679
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Tyrosinase is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. It's a melanocyte-specific enzyme critical for the synthesis of melanin, a process normally restricted to a post-Golgi compartment termed the melanosome. Loss-of-function mutations in tyrosinase are the cause of oculocutaneous albinism. The 60-kDa tyrosinase core polypeptide is modified in the endoplasmic reticulum (ER) by cotranslational addition of multiple N-linked glycans, producing the 70-kDa species. Complex sugar modifications in the Golgi apparatus further increases tyrosinase's molecular mass to 80 kDa, the size of the mature wild-type (WT) isoform. (PMID:10823941)

实验方案

Product Specific Protocols
WB protocol for Tyrosinase antibody 31291-1-APDownload protocol
IHC protocol for Tyrosinase antibody 31291-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols