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验证数据展示

  • WB analysis using 24601-1-AP (same clone as 24601-1-PBS)

    WB analysis using 24601-1-AP (same clone as 24601-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 24601-1-AP (Tuberin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24601-1-PBS in a different storage buffer formulation.

  • IP experiment of SH-SY5Y using 24601-1-AP (same clone as 24601-1-PBS)

    IP experiment of SH-SY5Y using 24601-1-AP (same clone as 24601-1-PBS)

    IP result of anti-Tuberin/TSC2 (IP:24601-1-AP, 4ug; Detection:24601-1-AP 1:1000) with SH-SY5Y cells lysate 1080 ug. This data was developed using the same antibody clone with 24601-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 24601-1-AP (same clone as 24601-1-PBS)

    IHC staining of mouse brain using 24601-1-AP (same clone as 24601-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24601-1-AP (Tuberin antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24601-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TSC2 antibody included
  • IHC staining of mouse brain using 24601-1-AP (same clone as 24601-1-PBS)

    IHC staining of mouse brain using 24601-1-AP (same clone as 24601-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24601-1-AP (Tuberin antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24601-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TSC2 antibody included
  • WB analysis of HeLa using 24601-1-AP (same clone as 24601-1-PBS)

    WB analysis of HeLa using 24601-1-AP (same clone as 24601-1-PBS)

    WB result of Tuberin/TSC2 antibody (24601-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Tuberin/TSC2 transfected HeLa cells. This data was developed using the same antibody clone with 24601-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 24601-1-AP (same clone as 24601-1-PBS)

    IF Staining of HEK-293 using 24601-1-AP (same clone as 24601-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using Tuberin/TSC2 antibody (24601-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 24601-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 24601-1-AP (same clone as 24601-1-PBS)

    FC experiment of HeLa using 24601-1-AP (same clone as 24601-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Tuberin (24601-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 24601-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

Tuberin/TSC2 Polyclonal antibody, PBS Only

Tuberin/TSC2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA
Cat No. 24601-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy TSC2 Ready-To-Use IHC Kit. TSC2 primary antibody included.

产品信息

24601-1-PBS targets Tuberin/TSC2 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag18133

Product name: Recombinant human TSC2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1436-1784 aa of BC150300

Sequence: RGYTISDSAPSRRGKRVERDALKSRATASNAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESQSFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVMVTPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRICEEAAYSNPSLPLVHPPSHSKAPAQTPAEPTPGYEVGQRKRLISSVEDFTEFV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tuberous sclerosis 2
别名
计算分子量 1807 aa, 201 kDa
观测分子量 200 kDa
GenBank蛋白编号BC150300
基因名称 TSC2
Gene ID (NCBI) 7249
RRIDAB_2879633
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP49815
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TSC2, also named as TSC4, FLJ43106 and LAM, acts as a GTPase-activating protein (GAP) for the small GTPase RHEB, a direct activator of the protein kinase activity of mTORC1. In complex with TSC1, TSC2 inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. TSC2 implicated as a tumor suppressor. It is involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling. TSC2 stimulates weakly the intrinsic GTPase activity of the Ras-related proteins RAP1A and RAB5 in vitro. Mutations in TSC2 lead to constitutive activation of RAP1A in tumors. Mutations in either TSC2 or the related TSC1 (hamartin) gene cause tuberous sclerosis complex (TSC), an autosomal dominant disorder characterized by development of multiple, widespread non-malignant tumors.

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