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验证数据展示

  • WB analysis using 33093-1-AP

    WB analysis using 33093-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 33093-1-AP (TrkB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 33093-1-AP

    IHC staining of mouse brain using 33093-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 33093-1-AP (TrkB antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 33093-1-AP

    IHC staining of mouse brain using 33093-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 33093-1-AP (TrkB antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using 33093-1-AP

    IF Staining of mouse brain using 33093-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using TrkB antibody (33093-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using 33093-1-AP

    IF Staining of mouse brain using 33093-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using TrkB antibody (33093-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TrkB Polyclonal antibody

TrkB Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 33093-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, IHC, IF-P, ELISA

别名

BDNF/NT-3 growth factors receptor, EC:2.7.10.1, GP145-TrkB/GP95-TrkB, Neurotrophic tyrosine kinase receptor type 2, Ntrk2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

33093-1-AP targets TrkB in WB, IHC, IF-P, ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 mouse, rat
免疫原

CatNo: Eg3000

Product name: Recombinant Mouse TrkB protein (rFc Tag) (HPLC verified)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 32-429 aa of NM_001025074.3

Sequence: CPTSCKCSSARIWCTEPSPGIVAFPRLEPNSVDPENITEILIANQKRLEIINEDDVEAYVGLRNLTIVDSGLKFVAYKAFLKNSNLRHINFTRNKLTSLSRRHFRHLDLSDLILTGNPFTCSCDIMWLKTLQETKSSPDTQDLYCLNESSKNMPLANLQIPNCGLPSARLAAPNLTVEEGKSVTLSCSVGGDPLPTLYWDVGNLVSKHMNETSHTQGSLRITNISSDDSGKQISCVAENLVGEDQDSVNLTVHFAPTITFLESPTSDHHWCIPFTVRGNPKPALQWFYNGAILNESKYICTKIHVTNHTEYHGCLQLDNPTHMNNGDYTLMAKNEYGKDERQISAHFMGRPGVDYETNPNYPEVLYEDWTTPTDIGDTTNKSNEIPSTDVADQSNREH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 neurotrophic tyrosine kinase, receptor, type 2
别名 BDNF/NT-3 growth factors receptor, EC:2.7.10.1, GP145-TrkB/GP95-TrkB, Neurotrophic tyrosine kinase receptor type 2, Ntrk2
计算分子量92 kDa
观测分子量90-95 kDa, 140-145 kDa
GenBank蛋白编号NM_001025074.3
基因名称 Ntrk2
Gene ID (NCBI) 18212
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP15209-1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Tropomyosin-related kinase B (TrkB) is a member of the neurotrophic tyrosine receptor kinase (NTRK) family. TrkB is primarily expressed in the central and peripheral nervous systems. TrkB plays an important role in neural development and maintenance through regulating neuronal differentiation, proliferation, survival, migration, neurogenesis, and plasticity.

实验方案

Product Specific Protocols
IF protocol for TrkB antibody 33093-1-APDownload protocol
IHC protocol for TrkB antibody 33093-1-APDownload protocol
WB protocol for TrkB antibody 33093-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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