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验证数据展示

  • IF Staining of HeLa using 83065-2-RR

    IF Staining of HeLa using 83065-2-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Tnfrsf11b antibody (83065-2-RR, Clone: 230325C3 ) at dilution of 1:150 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of RAW 264.7 using 83065-2-RR

    FC experiment of RAW 264.7 using 83065-2-RR

    1x10^6 RAW 264.7 cells were intracellularly stained with 0.25 ug Anti-Mouse Tnfrsf11b (83065-2-RR, Clone:230325C3) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Affinity and Kinetic Characterization of 83065-2-RR

    Affinity and Kinetic Characterization of 83065-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83065-2-RR against Mouse Tnfrsf11b were performed. The affinity constant is 18.1 pM.

Tnfrsf11b Recombinant monoclonal antibody

Tnfrsf11b Uni-rAb® Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 83065-2-RR

产品说明书

CloneNo. 230325C3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IF/ICC, FC (Intra), ELISA

别名

230325C3, OCIF, Opg, Osteoprotegerin, PDB5

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inRAW 264.7 cells

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83065-2-RR targets Tnfrsf11b in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Eg0598

Product name: Recombinant mouse Tnfrsf11b protein

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 22-401 aa of BC049782.1

Sequence: ETLPPKYLHYDPETGHQLLCDKCAPGTYLKQHCTVRRKTLCVPCPDHSYTDSWHTSDECVYCSPVCKELQSVKQECNRTHNRVCECEEGRYLEIEFCLKHRSCPPGSGVVQAGTPERNTVCKKCPDGFFSGETSSKAPCRKHTNCSTFGLLLIQKGNATHDNVCSGNREATQKCGIDVTLCEEAFFRFAVPTKIIPNWLSVLVDSLPGTKVNAESVERIKRRHSSQEQTFQLLKLWKHQNRDQEMVKKIIQDIDLCESSVQRHLGHANLTTEQLRALMESLPGKKISPEEIERTRKTCKSSEQLLKLLSLWRIKNGDQDTLKGLMYALKHLKTSHFPKTVTHSLRKTMRFLHSFTMYRLYQKLFLEMIGNQVQSVKISCL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 tumor necrosis factor receptor superfamily, member 11b (osteoprotegerin)
别名 230325C3, OCIF, Opg, Osteoprotegerin, PDB5
计算分子量46KD
GenBank蛋白编号BC049782.1
基因名称 Tnfrsf11b
Gene ID (NCBI) 18383
RRIDAB_3670789
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ6PI12
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for Tnfrsf11b antibody 83065-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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