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验证数据展示

  • WB analysis using 28005-1-AP (same clone as 28005-1-PBS)

    WB analysis using 28005-1-AP (same clone as 28005-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28005-1-AP (Tissue Factor antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28005-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 28005-1-AP (same clone as 28005-1-PBS)

    IHC staining of human stomach cancer using 28005-1-AP (same clone as 28005-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 28005-1-AP (Tissue Factor antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28005-1-PBS in a different storage buffer formulation.

Tissue Factor/CD142 Polyclonal antibody, PBS Only

Tissue Factor/CD142 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 28005-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

CD142, Coagulation factor III, F3, Tissue factor, TF

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

28005-1-PBS targets Tissue Factor/CD142 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag27553

Product name: Recombinant human F3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 113-251 aa of BC011029

Sequence: GNVESTGSAGEPLYENSPEFTPYLETNLGQPTIQSFEQVGTKVNVTVEDERTLVRRNNTFLSLRDVFGKDLIYTLYYWKSSSSGKKTAKTNTNEFLIDVDKGENYCFSVQAVIPSRTVNRKSTDSPVECMGQEKGEFRE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 coagulation factor III (thromboplastin, tissue factor)
别名 CD142, Coagulation factor III, F3, Tissue factor, TF
计算分子量 295 aa, 33 kDa
观测分子量45 kDa
GenBank蛋白编号BC011029
基因名称 F3
Gene ID (NCBI) 2152
RRIDAB_3669635
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP13726
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Normal blood coagulation is a complex process, involving a cascade of activation of different plasma proteins, ultimately resulting in the formation of a clot, called fibrin. Tissue Factor (TF), also named Coagulation factor III or F3, is the primary initiator of the blood coagulation cascade and plays an essential role in hemostasis (PMID: 26877187; PMID: 33796108).

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