验证数据展示
经过测试的应用
| Positive WB detected in | human placenta tissue, mouse brain tissue, rat brain tissue |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10858-1-AP targets Timp-3 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, pig |
| 免疫原 |
CatNo: Ag1253 Product name: Recombinant human Timp-3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-211 aa of BC014277 Sequence: MTPWLGLIVLLGSWSLGDWGAEACTCSPSHPQDAFCNSDIVIRAKVVGKKLVKEGPFGTLVYTIKQMKMYRGFTKMPHVQYIHTEASESLCGLKLEVNKYQYLLTGRVYDGKMYTGLCNFVERWDQLTLSQRKGLNYRYHLGCNCKIKSCYYLPCFVTSKNECLWTDMLSNFGYPGYQSKHYACIRQKGGYCSWYRGWAPPDKSIINATDP 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | TIMP metallopeptidase inhibitor 3 |
| 别名 | TIMP3, Protein MIG-5, Protein MIG 5, Metalloproteinase inhibitor 3, K222TA2 |
| 计算分子量 | 24 kDa |
| 观测分子量 | 20-30 kDa |
| GenBank蛋白编号 | BC014277 |
| 基因名称 | TIMP3 |
| Gene ID (NCBI) | 7078 |
| RRID | AB_2204973 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P35625 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Timp-3 (TIMP metallopeptidase inhibitor 3), also known as SFD. It is expected to be located in extracellular space, and the protein is enriched in the placenta tissue and fat tissue. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix (ECM). Expression of this gene is induced in response to mitogenic stimulation, and this netrin domain-containing protein is localized to the ECM. Mutations in this gene have been associated with the autosomal dominant disorder Sorsby's fundus dystrophy. TIMP-3 is expressed as an unglycosylated 24 kDa and glycosylated 29 kDa protein with inhibitory activity against interstitial collagenase, stromelysin-1, and gelatinases A and B (PMID:11827795).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for Timp-3 antibody 10858-1-AP | Download protocol |
| WB protocol for Timp-3 antibody 10858-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Comprehensive human proteome profiles across a 50-year lifespan reveal aging trajectories and signatures | ||
Theranostics miR-17-3p Contributes to Exercise-Induced Cardiac Growth and Protects against Myocardial Ischemia-Reperfusion Injury. | ||
Cell Mol Biol Lett Limbal niche cells can reduce the angiogenic potential of cultivated oral mucosal epithelial cells. | ||
Mater Today Bio Cationic microbubble loading hSIRT3 and hTIMP3 optimize cardiac-targeted delivery and myocardial protection in the porcine MI/R model. | ||
Br J Cancer KDM1A, a potent and selective target, for the treatment of DNMT3A-deficient non-small cell lung cancer | ||
Cell Prolif Methyltransferase-like 3 suppresses phenotypic switching of vascular smooth muscle cells by activating autophagosome formation |
