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验证数据展示

  • WB analysis of mouse lung using 19157-1-AP (same clone as 19157-1-PBS)

    WB analysis of mouse lung using 19157-1-AP (same clone as 19157-1-PBS)

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 19157-1-AP (Tie-2/CD202b antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19157-1-PBS in a different storage buffer formulation.

  • IF Staining of HUVEC using 19157-1-AP (same clone as 19157-1-PBS)

    IF Staining of HUVEC using 19157-1-AP (same clone as 19157-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using Tie-2/CD202b antibody (19157-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 19157-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 19157-1-AP (same clone as 19157-1-PBS)

    IHC staining of human placenta using 19157-1-AP (same clone as 19157-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 19157-1-AP (Tie2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19157-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 19157-1-AP (same clone as 19157-1-PBS)

    IHC staining of human placenta using 19157-1-AP (same clone as 19157-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 19157-1-AP (Tie2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19157-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 19157-1-AP (same clone as 19157-1-PBS)

    IHC staining of mouse kidney using 19157-1-AP (same clone as 19157-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 19157-1-AP (Tie2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19157-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse lung using 19157-1-AP (same clone as 19157-1-PBS)

    IP experiment of mouse lung using 19157-1-AP (same clone as 19157-1-PBS)

    IP result of anti-Tie-2/CD202b (IP:19157-1-AP, 4ug; Detection:19157-1-AP 1:1000) with mouse lung tissue lysate 1240 ug. This data was developed using the same antibody clone with 19157-1-PBS in a different storage buffer formulation.

Tie-2/CD202b Polyclonal antibody, PBS Only

Tie-2/CD202b Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 19157-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

TEK, Angiopoietin 1 receptor, Angiopoietin-1 receptor, CD202B, EC:2.7.10.1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

19157-1-PBS targets Tie-2/CD202b in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag13523

Product name: Recombinant human TEK protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 779-1124 aa of BC035514

Sequence: RRMAQAFQNVREEPAVQFNSGTLALNRKVKNNPDPTIYPVLDWNDIKFQDVIGEGNFGQVLKARIKKDGLRMDAAIKRMKEYASKDDHRDFAGELEVLCKLGHHPNIINLLGACEHRGYLYLAIEYAPHGNLLDFLRKSRVLETDPAFAIANSTASTLSSHHLLHFAADVARGMDYLSQKQFIHRDLAARNILVGENYVAKIADFGLSRGQEVYVKKTMGRLPVRWMAIESLNYSVYTTNSDVWSYGVLLWEIVSLGGTPYCGMTCAELYEKLPQGYRLEKPLNCDDEVYDLMRQCWREKPYERPSFAQILVSLNRMLEERKTYVNTTLYEKFTYAGIDCSAEEAA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 TEK tyrosine kinase, endothelial
别名 TEK, Angiopoietin 1 receptor, Angiopoietin-1 receptor, CD202B, EC:2.7.10.1
计算分子量 1124 aa, 126 kDa
观测分子量 140 kDa
GenBank蛋白编号BC035514
基因名称 Tie2
Gene ID (NCBI) 7010
RRIDAB_10644297
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ02763
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Tie2 (also known as TEK) is a tyrosine-protein kinase expressed almost exclusively on endothelial cells. It contains two immunoglobulin-like domains, three epidermal growth factor (EGF)--like domains and three fibronectin type III repeats. Tie2 acts as a cell-surface receptor for ANGPT1, ANGPT2, and ANGPT4 and regulates angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Mutations in the gene Tie2 are associated with inherited venous malformations of the skin and mucous membranes. Human Tie2 has a calculated molecular weight of 126 kDa. As a result of glycosylation, the apparent molecular mass of Tie2 is approximately 140-160 kDa.

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