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Thioredoxin Recombinant antibody

Thioredoxin Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

7G10

Cat No : 82765-1-RR

Print datasheet

Synonyms

ADF, ATL derived factor, DKFZp686B1993, SASP, thioredoxin, TRDX, TRX, TRX1, TXN

验证数据展示

  • WB analysis using 82765-1-RR

    WB analysis using 82765-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82765-1-RR (Thioredoxin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inA549 cells, HeLa cells, HepG2 cells, K-562 cells, MCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy TXN Ready-To-Use IHC Kit. TXN primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82765-1-RR targets Thioredoxin in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA
Tested Reactivity Human
Immunogen Thioredoxin fusion protein Ag6989 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name thioredoxin
Synonyms ADF, ATL derived factor, DKFZp686B1993, SASP, thioredoxin, TRDX, TRX, TRX1, TXN
Calculated Molecular Weight 12 kDa
Observed Molecular Weight 12 kDa
GenBank Accession NumberBC003377
Gene Symbol TXN
Gene ID (NCBI) 7295
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP10599
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TXN, TRDX, TRX, TRX1, ADF and SASP, belongs to the thioredoxin family. It participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. TXN plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. TXN induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.

实验方案

Product Specific Protocols
WB protocol for Thioredoxin antibody 82765-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols