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验证数据展示

  • WB analysis of mouse liver using 13595-1-AP (same clone as 13595-1-PBS)

    WB analysis of mouse liver using 13595-1-AP (same clone as 13595-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 13595-1-AP (Tenascin-X antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13595-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 13595-1-AP (same clone as 13595-1-PBS)

    IHC staining of human kidney using 13595-1-AP (same clone as 13595-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 13595-1-AP (Tenascin-X antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 13595-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse liver using 13595-1-AP (same clone as 13595-1-PBS)

    IP experiment of mouse liver using 13595-1-AP (same clone as 13595-1-PBS)

    IP result of anti-Tenascin-X (IP:13595-1-AP, 4ug; Detection:13595-1-AP 1:300) with mouse liver tissue lysate 4000ug. This data was developed using the same antibody clone with 13595-1-PBS in a different storage buffer formulation.

  • WB analysis using 13595-1-AP (same clone as 13595-1-PBS)

    WB analysis using 13595-1-AP (same clone as 13595-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13595-1-AP (Tenascin-X antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13595-1-PBS in a different storage buffer formulation.

Tenascin-X Polyclonal antibody, PBS Only

Tenascin-X Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 13595-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

13595-1-PBS targets Tenascin-X in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag4501

Product name: Recombinant human TNXB protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 321-673 aa of BC033740

Sequence: PGSAVDYPLHDLVLHTNYTATVRGLRGPNLTSPASITFTTGLEAPRDLEAKEVTPRTALLTWTEPPVRPAGYLLSFHTPGGQNQEILLPGGITSHQLLGLFPSTSYNARLQAMWGQSLLPPVSTSFTTGGLRIPFPRDCGEEMQNGAGASRTSTIFLNGNRERPLNVFCDMETDGGGWLVFQRRMDGQTDFWRDWEDYAHGFGNISGEFWLGNEALHSLTQAGDYSMRVDLRAGDEAVFAQYDSFHVDSAAEYYRLHLEGYHGTAGDSMSYHSGSVFSARDRDPNSLLISCAVSYRGAWWYRNCHYANLNGLYGSTVDHQGVSWYHWKGFEFSVPFTEMKLRPRNFRSPAGGG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tenascin XB
别名
计算分子量 4289 aa, 464 kDa
观测分子量 75 kDa, 140 kDa
GenBank蛋白编号BC033740
基因名称 TNXB
Gene ID (NCBI) 7148
RRIDAB_10644124
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP22105
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Tenascin-X (TNXB) is an extracellular matrix glycoprotein predominantly located in the outer reticular lamina of the basement membrane (BM) (PMID: 23768946). It interacts with many other ECM proteins and it accelerates collagen fibrillogenesis in vitro. TNXB plays a role in interactions between cell and ECM, antiadhesive effect, inhibiting cell migration, and in maintaining homeostasis of the extracellular matrix. Deficiency of TNXB has been associated with the connective tissue disorder Ehlers-Danlos syndrome. This antibody is raised against human TNXB and detects a band of about 75 kDa, which probably represents a proteolytic cleaved form of human TNXB (PMID: 17263730). But the serum form of tenascin-X is a 140-kD protein probably resulting from proteolytic cleavage or alternative splicing of tenascin-X(PMID: 16567571, 11642233).

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