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Talin-1 Recombinant antibody

Talin-1 Recombinant Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

7D11

Cat No : 82856-4-RR

Print datasheet

Synonyms

ILWEQ, KIAA1027, talin 1, TLN, TLN1

验证数据展示

  • WB analysis using 82856-4-RR

    WB analysis using 82856-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82856-4-RR (Talin-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of A549 using 82856-4-RR

    IF Staining of A549 using 82856-4-RR

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using Talin-1 antibody (82856-4-RR, Clone: 7D11 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells, K-562 cells, MCF-7 cells, NIH/3T3 cells, C6 cells
Positive IF detected inA549 cells, HUVEC cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82856-4-RR targets Talin-1 in WB, IF, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications IF/ICC, WB, ELISA
Tested Reactivity Human, Mouse, Rat
Immunogen Talin-1 fusion protein Ag5367 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name talin 1
Synonyms ILWEQ, KIAA1027, talin 1, TLN, TLN1
Calculated Molecular Weight 2541 aa, 270 kDa
Observed Molecular Weight270 kDa
GenBank Accession NumberBC042923
Gene Symbol TLN1
Gene ID (NCBI) 7094
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y490
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Talin-1 is a large cytoskeletal protein consisting of a globular head domain and a long rod comprised of a series of α-helical bundles (PMID: 35266014). Talin-1 is ubiquitously expressed and is a major component of focal adhesions which are multi-protein, integrin-containing complexes, mediating cell migration, adhesion and integrin signalling (PMID: 33431906). Talin-1 also plays a role in tumor formation, migration and metastasis in various malignancies (PMID: 37013489).

实验方案

Product Specific Protocols
WB protocol for Talin-1 antibody 82856-4-RRDownload protocol
IF protocol for Talin-1 antibody 82856-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols