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验证数据展示

  • WB analysis using 29213-1-AP

    WB analysis using 29213-1-AP

    U-251 cell lysates were subjected to SDS PAGE followed by western blot with 29213-1-AP (TYW3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 29213-1-AP

    IF Staining of U2OS using 29213-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using TYW3 antibody (29213-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

TYW3 Polyclonal antibody

TYW3 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 29213-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, IF/ICC, ELISA

别名

C1orf171, FLJ40918, tRNA yW synthesizing protein 3, TYW3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inU-251 cells
Positive IF/ICC detected inU2OS cells
Planning an IHC experiment? We recommend our IHCeasy TYW3 Ready-To-Use IHC Kit. TYW3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29213-1-AP targets TYW3 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human
免疫原 TYW3 fusion protein Ag30156 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tRNA-yW synthesizing protein 3 homolog (S. cerevisiae)
别名 C1orf171, FLJ40918, tRNA yW synthesizing protein 3, TYW3
计算分子量 30 kDa
GenBank蛋白编号NM_138467
基因名称 TYW3
Gene ID (NCBI) 127253
RRIDAB_2918250
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6IPR3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TYW3 antibody 29213-1-APDownload protocol
IF protocol for TYW3 antibody 29213-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols