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验证数据展示

  • WB analysis using 28513-1-AP (same clone as 28513-1-PBS)

    WB analysis using 28513-1-AP (same clone as 28513-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28513-1-AP (TYRO3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28513-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 28513-1-AP (same clone as 28513-1-PBS)

    IHC staining of human colon cancer using 28513-1-AP (same clone as 28513-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28513-1-AP (TYRO3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28513-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TYRO3 antibody included
  • IF Staining of HCT 116 using 28513-1-AP (same clone as 28513-1-PBS)

    IF Staining of HCT 116 using 28513-1-AP (same clone as 28513-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HCT 116 cells using TYRO3 antibody (28513-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 28513-1-PBS in a different storage buffer formulation.

TYRO3 Polyclonal antibody, PBS Only

TYRO3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 28513-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Brt, BYK, Dtk, EC:2.7.10.1, RSE

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy TYRO3 Ready-To-Use IHC Kit. TYRO3 primary antibody included.

产品信息

28513-1-PBS targets TYRO3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag29540

Product name: Recombinant human TYRO3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 451-596 aa of BC049368

Sequence: LRKRRKETRFGQAFDSVMARGEPAVHFRAARSFNRERPERIEATLDSLGISDELKEKLEDVLIPEQQFTLGRMLGKGEFGSVREAQLKQEDSSFVKVAVKMLKADIIASSDIEEFLREAACMKEFDHPHVAKLVGVSLRSRAKGRL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 TYRO3 protein tyrosine kinase
别名 Brt, BYK, Dtk, EC:2.7.10.1, RSE
计算分子量 97 kDa
观测分子量 140 kDa
GenBank蛋白编号BC049368
基因名称 TYRO3
Gene ID (NCBI) 7301
RRIDAB_2881162
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ06418
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TYRO3 has a structure includes a cytoplasmic domain and an extracellular ligand binding region. And ligand binding at the cell surface induces dimerization and autophosphorylation of TYRO3 on its intracellular domain that provides docking sites for downstream signaling molecules. TYRO3 is associated with many physiological processes including cell survival, migration and differentiation. It has been reported that TYRO3 can be expressed in various regions of the mammalian central nervous system. 28513-1-AP antibody recognizes the 97 kDa and 140 kDa protein which might be the glycosylated form similar to the papers.((PMID: 8873131, 23354874, 21539887)

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