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Thymidylate synthase Monoclonal antibody

Thymidylate synthase Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 66725-1-Ig

产品说明书

CloneNo. 1F5B7

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), ELISA and More (1)

TYMS, 1F5B7, DKCD, EC:2.1.1.45, HsT422

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存


经过测试的应用

Positive WB detected inHEK-293 cells, HepG2 cells, Jurkat cells
Positive IHC detected inhuman pancreas cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy TYMS Ready-To-Use IHC Kit. TYMS primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66725-1-Ig targets Thymidylate synthase in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
文献引用应用WB, IF
经测试反应性 human
文献引用反应性human
免疫原 Thymidylate synthase fusion protein Ag7027 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 thymidylate synthetase
别名 TYMS, 1F5B7, DKCD, EC:2.1.1.45, HsT422
计算分子量 36 kDa
观测分子量 36 kDa
GenBank蛋白编号BC002567
基因名称 TYMS
Gene ID (NCBI) 7298
RRIDAB_2882076
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP04818
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Thymidylate synthase gene (TYMS) encodes thymidylate synthase (TS) which is an important factor in the growth of tumor cells. TS can catalyze the transformation of intracellular uridine monophosphate (dump) into dTMP which is required for DNA replication and repairing. TS is a key enzyme in the process of cell proliferation, also is the important target enzymes of 5-FU and other chemotherapy drugs. The expression of TYMS is negatively correlated with the efficacy of chemotherapy and the prognosis of patients who suffer from rectal cancer, breast cancer, colorectal cancer, gastric cancer, head and neck cancer, esophageal cancer, pancreatic cancer and so on.

实验方案

Product Specific Protocols
WB protocol for Thymidylate synthase antibody 66725-1-IgDownload protocol
IHC protocol for Thymidylate synthase antibody 66725-1-IgDownload protocol
IF protocol for Thymidylate synthase antibody 66725-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Med Chem

Fuplatin: An Efficient and Low-Toxic Dual-Prodrug

Authors - Ran Zhang
humanWB

Biochem Pharmacol

Design, synthesis and biological evaluation of novel curcumin-fluorouracil hybrids as potential anti-cancer agents

Authors - Xiaotong Wang
humanIF

Front Immunol

Single-cell sequencing analysis reveals cancer-associated pericyte subgroup in esophageal squamous cell carcinoma to predict prognosis

Authors - Kai Xiong
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