验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HEK-293 cells, K-562 cells, MJ cells, Jurkat cells, MOLT-4 cells, TF-1 cells, HSC-T6 cells, A549 cells, THP-1 cells |
| Positive IHC detected in | human colon cancer tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy TYK2 Ready-To-Use IHC Kit. TYK2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 13 publications below |
| IHC | See 1 publications below |
产品信息
67411-1-Ig targets TYK2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | Human, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag9442 Product name: Recombinant human TYK2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 901-1187 aa of BC014243 Sequence: RDLGEGHFGKVSLYCYDPTNDGTGEMVAVKALKADCGPQHRSGWKQEIDILRTLYHEHIIKYKGCCEDQGEKSLQLVMEYVPLGSLRDYLPRHSIGLAQLLLFAQQICEGMAYLHSQHYIHRDLAARNVLLDNDRLVKIGDFGLAKAVPEGHEYYRVREDGDSPVFWYAPECLKEYKFYYASDVWSFGVTLYELLTHCDSSQSPPTKFLELIGIAQGQMTVLRLTELLERGERLPRPDKCPCEVYHLMKNCWETEASFRPTFENLIPILKTVHEKYQGQAPSVFSVC 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | tyrosine kinase 2 |
| 别名 | JTK1, TYK2, tyrosine kinase 2 |
| 计算分子量 | 1187 aa, 134 kDa |
| 观测分子量 | 134 kDa |
| GenBank蛋白编号 | BC014243 |
| 基因名称 | TYK2 |
| Gene ID (NCBI) | 7297 |
| RRID | AB_2882652 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P29597 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for TYK2 antibody 67411-1-Ig | Download protocol |
| IHC protocol for TYK2 antibody 67411-1-Ig | Download protocol |
| WB protocol for TYK2 antibody 67411-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Lett HDAC4 mediated LHPP deacetylation enhances its destabilization and promotes the proliferation and metastasis of nasopharyngeal carcinoma | ||
Antioxidants (Basel) Transcriptotype-Driven Discovery of Apigenin as a Therapy against Cholestatic Liver Fibrosis: Through Inhibition of PANoptosis and Following Type-I Interferon Responses | ||
Chin J Nat Med Picroside II promotes HSC apoptosis and inhibits the cholestatic liver fibrosis in Mdr2-/- mice by polarizing M1 macrophages and balancing immune responses | ||
J Med Chem Discovery of a Highly Potent and Selective Tyrosine Kinase 2 (TYK2) Degrader with In Vivo Therapeutic Efficacy in a Murine Psoriasis Model |
