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TYK2 Polyclonal antibody

TYK2 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 16412-1-AP

Print datasheet

Synonyms

JTK1, TYK2, tyrosine kinase 2

验证数据展示

  • WB analysis using 16412-1-AP

    WB analysis using 16412-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16412-1-AP (TYK2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of A549 using 16412-1-AP

    IF Staining of A549 using 16412-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using TYK2 antibody (16412-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human liver cancer using 16412-1-AP

    IHC staining of human liver cancer using 16412-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16412-1-AP (TYK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 16412-1-AP

    IHC staining of human liver cancer using 16412-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16412-1-AP (TYK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inJurkat cells, RAW 264.7 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

16412-1-AP targets TYK2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF, IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen TYK2 fusion protein Ag9462 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tyrosine kinase 2
Synonyms JTK1, TYK2, tyrosine kinase 2
Calculated Molecular Weight 1187 aa, 134 kDa
Observed Molecular Weight134 kDa
GenBank Accession NumberBC014243
Gene Symbol TYK2
Gene ID (NCBI) 7297
RRIDAB_2918047
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP29597
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TYK2 antibody 16412-1-APDownload protocol
IHC protocol for TYK2 antibody 16412-1-APDownload protocol
IF protocol for TYK2 antibody 16412-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Biomed Pharmacother

JAK1/JAK2 degraders based on PROTAC for topical treatment of atopic dermatitis

Authors - Junchao Wu
View Article