验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, RAW 264.7 cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy TYK2 Ready-To-Use IHC Kit. TYK2 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 4 publications below |
| IF | See 1 publications below |
产品信息
16412-1-AP targets TYK2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag9462 Product name: Recombinant human TYK2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 901-1187 aa of BC014243 Sequence: RDLGEGHFGKVSLYCYDPTNDGTGEMVAVKALKADCGPQHRSGWKQEIDILRTLYHEHIIKYKGCCEDQGEKSLQLVMEYVPLGSLRDYLPRHSIGLAQLLLFAQQICEGMAYLHSQHYIHRDLAARNVLLDNDRLVKIGDFGLAKAVPEGHEYYRVREDGDSPVFWYAPECLKEYKFYYASDVWSFGVTLYELLTHCDSSQSPPTKFLELIGIAQGQMTVLRLTELLERGERLPRPDKCPCEVYHLMKNCWETEASFRPTFENLIPILKTVHEKYQGQAPSVFSVC 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | tyrosine kinase 2 |
| 别名 | EC:2.7.10.2, JTK1, tyrosine kinase 2 |
| 计算分子量 | 1187 aa, 134 kDa |
| 观测分子量 | 134 kDa |
| GenBank蛋白编号 | BC014243 |
| 基因名称 | TYK2 |
| Gene ID (NCBI) | 7297 |
| RRID | AB_2918047 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P29597 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for TYK2 antibody 16412-1-AP | Download protocol |
| WB protocol for TYK2 antibody 16412-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Biomed Pharmacother JAK1/JAK2 degraders based on PROTAC for topical treatment of atopic dermatitis | ||
Dev Cell Secreted PTEN binds PLXDC2 on macrophages to drive antitumor immunity and tumor suppression | ||
J Biol Chem Elongator is required for pattern recognition receptor and type I interferon signaling in macrophages. | ||
Inflamm Res IL-22/IL-22R signaling attenuates mitochondrial damage in PCOS by modulating granulosa cell lipid metabolism through ETS1. | ||
J Cosmet Dermatol Dual-Targeting Oligopeptide-215 Regulates Skin Barrier Homeostasis Through Concurrent Modulation of JAK-STAT and NF-κB Signaling. |
