验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, HeLa cells, HepG2 cells, HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
85424-1-RR targets TXNRD2 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | TXNRD2 fusion protein Ag8367 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | thioredoxin reductase 2 |
| 别名 | TRXR2, 242912F11, EC:1.8.1.9, KIAA1652, Selenoprotein Z |
| 计算分子量 | 524 aa, 56 kDa |
| 观测分子量 | 54 kDa |
| GenBank蛋白编号 | BC007489 |
| 基因名称 | TXNRD2 |
| Gene ID (NCBI) | 10587 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9NNW7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Human mitochondrial thioredoxin reductase (TXNRD2) is a selenocysteine-containing enzyme essential for mitochondrial oxygen radical scavenging. Three mammalian thioredoxin reductases exist; a cytosolic (TXNRD1), a mitochondrial (TXNRD2), and a testis-specific thioredoxin reductase (TXNRD3). (PMID: 21247928, PMID: 26199228)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TXNRD2 antibody 85424-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |