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验证数据展示

  • WB analysis using 67728-1-Ig (same clone as 67728-1-PBS)

    WB analysis using 67728-1-Ig (same clone as 67728-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67728-1-Ig (TXNRD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67728-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67728-1-Ig (same clone as 67728-1-PBS)

    IHC staining of human liver cancer using 67728-1-Ig (same clone as 67728-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67728-1-Ig (TXNRD1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67728-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TXNRD1 antibody included
  • IF Staining of HeLa using 67728-1-Ig (same clone as 67728-1-PBS)

    IF Staining of HeLa using 67728-1-Ig (same clone as 67728-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TXNRD1 antibody (67728-1-Ig, Clone: 1B10C4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67728-1-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP51846-1

    Cytometric bead array sample test of MP51846-1

    Cytometric bead array sample test of MP51846-1, TXNRD1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67728-1-PBS. Detection antibody: 67728-2-PBS.

  • Cytometric bead array standard curve of MP51846-1

    Cytometric bead array standard curve of MP51846-1

    Cytometric bead array standard curve of MP51846-1, TXNRD1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67728-1-PBS. Detection antibody: 67728-2-PBS. Standard:Ag30071. Range: 0.781-100 ng/mL.

  • Featured Product
  • KD/KO Validated

TXNRD1 Monoclonal antibody, PBS Only (Capture)

TXNRD1 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 67728-1-PBS

产品说明书

CloneNo. 1B10C4

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

别名

TRXR1, 1B10C4, EC:1.11.1.2, EC:1.8.1.9, Gene associated with retinoic and IFN-induced mortality 12 protein

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy TXNRD1 Ready-To-Use IHC Kit. TXNRD1 primary antibody included.

产品信息

67728-1-PBS targets TXNRD1 as part of a matched antibody pair:

MP51846-1: 67728-1-PBS capture and 67728-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag30071

Product name: Recombinant human TXNRD1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 199-500 aa of BC018122

Sequence: SYVALECAGFLAGIGLDVTVMVRSILLRGFDQDMANKIGEHMEEHGIKFIRQFVPIKVEQIEAGTPGRLRVVAQSTNSEEIIEGEYNTVMLAIGRDACTRKIGLETVGVKINEKTGKIPVTDEEQTNVPYIYAIGDILEDKVELTPVAIQAGRLLAQRLYAGSTVKCDYENVPTTVFTPLEYGACGLSEEKAVEKFGEENIEVYHSYFWPLEWTIPSRDNNKCYAKIICNTKDNERVVGFHVLGPNAGEVTQGFAAALKCGLTKKQLDSTIGIHPVCAEVFTTLSVTKRSGASILQAGCUG

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 thioredoxin reductase 1
别名 TRXR1, 1B10C4, EC:1.11.1.2, EC:1.8.1.9, Gene associated with retinoic and IFN-induced mortality 12 protein
计算分子量 55 kDa
观测分子量50-70 kDa
GenBank蛋白编号BC018122
基因名称 TXNRD1
Gene ID (NCBI) 7296
RRIDAB_2882914
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ16881
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Thioredoxin reductase-1 (TXNRD1) is uniquely capable of utilizing electrons from NADPH to recover the reduced state of TRX1. Interestingly, TXNRD1 is upregulated in many human malignancies and promotes cancer progression, and attenuation of TXNRD1 levels effectively suppresses the growth of tumor cells (PMID: 31384178). TXNRD1 is also upregulated in many human malignancies and functions as a prognostic factor for many tumors, such as oral squamous cell carcinomas, lung cancer, breast cancer, and astrocytomas. TXNRD1 protein levels were analyzed by inmmunoblotting. Bands of ~55 kDa for TXNRD1 were observed. IHC analysis suggested TXNRD1 was mainly located in the cytoplasm. (PMID: 28536696, PMID: 20584310)

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