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验证数据展示

  • WB analysis of K-562 using 15289-1-AP

    WB analysis of K-562 using 15289-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 15289-1-AP (TXNL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of HL-60 using 15289-1-AP

    IP experiment of HL-60 using 15289-1-AP

    IP result of anti-TXNL1 (IP:15289-1-AP, 4ug; Detection:15289-1-AP 1:300) with HL-60 cells lysate 960ug.

  • IHC staining of human heart using 15289-1-AP

    IHC staining of human heart using 15289-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15289-1-AP (TXNL1 Antibody) at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with TXNL1 antibody included
  • IHC staining of human heart using 15289-1-AP

    IHC staining of human heart using 15289-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15289-1-AP (TXNL1 Antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with TXNL1 antibody included
  • IF Staining of RAW 264.7 using 15289-1-AP

    IF Staining of RAW 264.7 using 15289-1-AP

    Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using 15289-1-AP (TXNL1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

TXNL1 Polyclonal antibody

TXNL1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 15289-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

TRP32, TXL, Thioredoxin-like protein 1, Thioredoxin like protein 1, thioredoxin like 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inK-562 cells, HL-60 cells
Positive IP detected inHL-60 cells
Positive IHC detected inhuman heart tissue, human thyroid cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inRAW 264.7 cells
Planning an IHC experiment? We recommend our IHCeasy TXNL1 Ready-To-Use IHC Kit. TXNL1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15289-1-AP targets TXNL1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 TXNL1 fusion protein Ag7481 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 thioredoxin-like 1
别名 TRP32, TXL, Thioredoxin-like protein 1, Thioredoxin like protein 1, thioredoxin like 1
计算分子量 32 kDa
观测分子量 32 kDa
GenBank蛋白编号BC001156
基因名称 TXNL1
Gene ID (NCBI) 9352
RRIDAB_2288008
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO43396
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TXNL1(Thioredoxin-like protein 1) is also named as TRP32, TXL, TXNL and belongs to the thioredoxin family. It might be involved in the cellular response to sugar starvation stress. The predicted 289-amino acid, 32.3-kD protein has 2 distinct domains: an N-terminal domain, which is 43% identical to human TXN, and a C-terminal domain, which showed no homology to other proteins in the sequence databases(PMID:9473519).TXNL1 functions as an effector of oxidants or redox sensor that couples oxidative stress to endocytosis, by converting redox changes into a specific GDI:Rab5-mediated endocytic response(PMID:17987124).

实验方案

Product Specific Protocols
WB protocol for TXNL1 antibody 15289-1-APDownload protocol
IHC protocol for TXNL1 antibody 15289-1-APDownload protocol
IF protocol for TXNL1 antibody 15289-1-APDownload protocol
IP protocol for TXNL1 antibody 15289-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Cell Death Dis

TXNL1-XRCC1 pathway regulates cisplatin-induced cell death and contributes to resistance in human gastric cancer.

Authors - W Xu
View Article
humanWB

Curr Cancer Drug Targets

TXNL1 induces apoptosis in cisplatin resistant human gastric cancer cell lines.

Authors - Pan Ni
View Article
WB

Vet Res

Newcastle disease virus V protein inhibits apoptosis in DF-1 cells by downregulating TXNL1.

Authors - Caiying Wang
View Article