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TXNIP Polyclonal antibody

TXNIP Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 27429-1-AP

Print datasheet

Synonyms

EST01027, HHCPA78, THIF, Thioredoxin binding protein 2, TXNIP, VDUP1

验证数据展示

  • WB analysis using 27429-1-AP

    WB analysis using 27429-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27429-1-AP (TXNIP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse colon using 27429-1-AP

    IHC staining of mouse colon using 27429-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 27429-1-AP (TXNIP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse colon using 27429-1-AP

    IHC staining of mouse colon using 27429-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 27429-1-AP (TXNIP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 27429-1-AP

    IF Staining of HeLa using 27429-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using TXNIP antibody (27429-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells, RAW 264.7 cells
Positive IHC detected inmouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27429-1-AP targets TXNIP in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Tested Reactivity human, mouse
Immunogen TXNIP fusion protein Ag26579 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name thioredoxin interacting protein
Synonyms EST01027, HHCPA78, THIF, Thioredoxin binding protein 2, TXNIP, VDUP1
Calculated Molecular Weight 391 aa, 44 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC093702
Gene Symbol TXNIP
Gene ID (NCBI) 10628
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H3M7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TXNIP, also known as VDUP-1 or TBP-2, belongs to alpha-arrestin protein family and is perhaps the only family member known to bind thioredoxin (TRX). TXNIP was induced by Vitamin D3, but not induced by another monocyte or macrophage differentiation inducer: phorbol 12-myristate 13-acetate (PMA). TXNIP bound catalytic active center of thioredoxin (TRX), which protected cells against oxidative stress. TXNIP was found to be a negative regulator of thioredoxin activity and inducer of the intracellular level of reactive oxygen species (ROS). TXNIP plays an important role in a wide variety of biological functions, such as the regulation of cell death, growth, differentiation, and energy metabolism.

实验方案

Product Specific Protocols
WB protocol for TXNIP antibody 27429-1-APDownload protocol
IHC protocol for TXNIP antibody 27429-1-APDownload protocol
IF protocol for TXNIP antibody 27429-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols