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验证数据展示

  • WB analysis using 13586-1-AP

    WB analysis using 13586-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13586-1-AP (TXNDC3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse testis using 13586-1-AP

    IP experiment of mouse testis using 13586-1-AP

    IP result of anti-TXNDC3 (IP:13586-1-AP, 4ug; Detection:13586-1-AP 1:1000) with mouse testis tissue lysate 4000ug.

TXNDC3 Polyclonal antibody

TXNDC3 Polyclonal Antibody for WB, IP, ELISA
Cat No. 13586-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, ELISA

别名

CILD6, NME8, Sptrx 2, SPTRX2, TXNDC3

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse testis tissue, human testis tissue, rat testis tissue
Positive IP detected inmouse testis tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13586-1-AP targets TXNDC3 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag4514

Product name: Recombinant human TXNDC3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 7-370 aa of BC036816

Sequence: EVQLQTVINNQSLWDEMLQNKGLTVIDVYQAWCGPCRAMQPLFRKLKNELNEDEILHFAVAEADNIVTLQPFRDKCEPVFLFSVNGKIIEKIQGANAPLVNKKVINLIDEERKIAAGEMARPQYPEIPLVDSDSEVSEESPCESVQELYSIAIIKPDAVISKKVLEIKRKITKAGFIIEAEHKTVLTEEQVVNFYSRIADQRDFEEFVSFMTSGLSYILVVSQGSKHNPPSEETEPQTDTEPNERSEDQPEVEAQVTPGMMKNKQDSLQEYLERQHLAQLCDIEEDAANVAKFMDAFFPDFKKMKSMKLEKTLALLRPNLFHERKDDVLRIIKDEDFKILEQRQVVLSEKEAQALCKEYENEDY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 thioredoxin domain containing 3 (spermatozoa)
别名 CILD6, NME8, Sptrx 2, SPTRX2, TXNDC3
计算分子量 588 aa, 67 kDa
观测分子量 67 kDa
GenBank蛋白编号BC036816
基因名称 TXNDC3
Gene ID (NCBI) 51314
RRIDAB_2212304
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N427
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IP protocol for TXNDC3 antibody 13586-1-APDownload protocol
WB protocol for TXNDC3 antibody 13586-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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