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TXNDC17 Polyclonal antibody

TXNDC17 Polyclonal Antibody for WB, IP, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF/ICC, ELISA

Conjugate

Unconjugated

Cat No : 20811-1-AP

Print datasheet

Synonyms

Protein 42 9 9, Thioredoxin like protein 5, TRP14, TXNDC17, TXNL5

验证数据展示

  • IP experiment of HEK-293 using 20811-1-AP

    IP experiment of HEK-293 using 20811-1-AP

    IP result of anti-TXNDC17 (IP:20811-1-AP, 4ug; Detection:20811-1-AP 1:1000) with HEK-293 cells lysate 2360 ug.

  • WB analysis of Jurkat using 20811-1-AP

    WB analysis of Jurkat using 20811-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 20811-1-AP (TXNDC17 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 20811-1-AP

    IF Staining of HeLa using 20811-1-AP

    Immunofluorescent analysis of Hela cells, using TXNDC17 antibody 20811-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

经过测试的应用

Positive WB detected inJurkat cells, HEK-293 cells, HeLa cells, human kidney tissue, human uterus tissue, K-562 cells, L02 cells, mouse pancreas tissue
Positive IP detected inHEK-293 cells
Positive IF detected inHela cells, HepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

20811-1-AP targets TXNDC17 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IF/ICC, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen TXNDC17 fusion protein Ag14766 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name thioredoxin domain containing 17
Synonyms Protein 42 9 9, Thioredoxin like protein 5, TRP14, TXNDC17, TXNL5
Calculated Molecular Weight 123 aa, 14 kDa
Observed Molecular Weight 14 kDa
GenBank Accession NumberBC006405
Gene Symbol TXNDC17
Gene ID (NCBI) 84817
RRIDAB_10695499
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BRA2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TXNDC17 antibody 20811-1-APDownload protocol
IF protocol for TXNDC17 antibody 20811-1-APDownload protocol
IP protocol for TXNDC17 antibody 20811-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Int J Radiat Biol

Combination of podophyllotoxin and rutin modulate radiation-induced alterations of jejunal proteome in mice.

Authors - Sania Bajaj
View Article