验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HEK-293 cells, HeLa cells, human kidney tissue, human uterus tissue, Jurkat cells, K-562 cells, L02 cells, mouse pancreas tissue |
| Positive IP detected in | HEK-293 cells |
| Positive IF/ICC detected in | HepG2 cells, HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
20811-1-AP targets TXNDC17 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | mouse |
| 免疫原 |
CatNo: Ag14766 Product name: Recombinant human TXNDC17 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-123 aa of BC006405 Sequence: MARYEEVSVSGFEEFHRAVEQHNGKTIFAYFTGSKDAGGKSWCPDCVQAEPVVREGLKHISEGCVFIYCQVGEKPYWKDPNNDFRKNLKVTAVPTLLKYGTPQKLVESECLQANLVEMLFSED 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | thioredoxin domain containing 17 |
| 别名 | TXNL5, TRP14, Thioredoxin-like protein 5, Thioredoxin domain-containing protein 17, Protein 42-9-9 |
| 计算分子量 | 123 aa, 14 kDa |
| 观测分子量 | 14 kDa |
| GenBank蛋白编号 | BC006405 |
| 基因名称 | TXNDC17 |
| Gene ID (NCBI) | 84817 |
| RRID | AB_10695499 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BRA2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TXNDC17 also known as TRP14, TXNL5, belongs to the thioredoxin family. TRP14 is a widely expressed, 123-amino acid protein with a WCPDC motif. TXNDC17 has a thioredoxin fold and a TRX-like active-site sequence(PMID: 14607844, 24778250).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for TXNDC17 antibody 20811-1-AP | Download protocol |
| IP protocol for TXNDC17 antibody 20811-1-AP | Download protocol |
| WB protocol for TXNDC17 antibody 20811-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
