• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of THP-1 using 22784-1-AP

    WB analysis of THP-1 using 22784-1-AP

    THP-1 cells were subjected to SDS PAGE followed by western blot with 22784-1-AP (TWISTNB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis using 22784-1-AP

    WB analysis using 22784-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22784-1-AP (TWISTNB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 22784-1-AP

    IHC staining of human ovary cancer using 22784-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 22784-1-AP (TWISTNB antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TWISTNB Polyclonal antibody

TWISTNB Polyclonal Antibody for WB, IHC, ELISA
Cat No. 22784-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

TWIST neighbor, RPA43, RNA polymerase I subunit F, POLR1F, DNA-directed RNA polymerase I subunit RPA43

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, THP-1 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22784-1-AP targets TWISTNB in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag18783

Product name: Recombinant human TWISTNB protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-320 aa of BC130298

Sequence: MAAGCSEAPRPAAASDGSLVGQAGVLPCLELPTYAAACALVNSRYSCLVAGPHQRHIALSPRYLNRKRTGIREQLDAELLRYSESLLGVPIAYDNIKVVGELGDIYDDQGHIHLNIEADFVIFCPEPGQKLMGIVNKVSSSHIGCLVHGCFNASIPKPEQLSAEQWQTMEINMGDELEFEVFRLDSDAAGVFCIRGKLNITSLQFKRSEVSEEVTENGTEEAAKKPKKKKKKKDPETYEVDSGTTKLADDADDTPMEESALQNTNNANGIWEEEPKKKKKKKKHQEVQDQDPVFQGSDSSGYQSDHKKKKKKRKHSEEAE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 TWIST neighbor
别名 TWIST neighbor, RPA43, RNA polymerase I subunit F, POLR1F, DNA-directed RNA polymerase I subunit RPA43
计算分子量 338 aa, 37 kDa
观测分子量 40-50 kDa
GenBank蛋白编号BC130298
基因名称 TWISTNB
Gene ID (NCBI) 221830
RRIDAB_2879167
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ3B726
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for TWISTNB antibody 22784-1-APDownload protocol
WB protocol for TWISTNB antibody 22784-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat Able
正在加载，请稍候...