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TUFM Polyclonal antibody

TUFM Polyclonal Antibody for FC, IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

FC, IF/ICC, WB, ELISA

Conjugate

Unconjugated

Cat No : 26730-1-AP

Print datasheet

Synonyms

COXPD4, EF Tu, EF TuMT, EFTU, P43, TUFM

验证数据展示

  • WB analysis using 26730-1-AP

    WB analysis using 26730-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26730-1-AP (TUFM antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 26730-1-AP

    IF Staining of HepG2 using 26730-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 26730-1-AP (TUFM antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of HepG2 using 26730-1-AP

    IF Staining of HepG2 using 26730-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TUFM antibody (26730-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • IF Staining of HepG2 using 26730-1-AP

    IF Staining of HepG2 using 26730-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TUFM antibody (26730-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of HepG2 using 26730-1-AP

    FC experiment of HepG2 using 26730-1-AP

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human TUFM (26730-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inA431 cells, Jurkat cells, mouse brain tissue, rat brain tissue
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 4 publications below

产品信息

26730-1-AP targets TUFM in WB, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IF/ICC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityrat, zebrafish
Immunogen TUFM fusion protein Ag24981 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Tu translation elongation factor, mitochondrial
Synonyms COXPD4, EF Tu, EF TuMT, EFTU, P43, TUFM
Calculated Molecular Weight 50 kDa
Observed Molecular Weight 46-50 kDa
GenBank Accession NumberBC010041
Gene Symbol TUFM
Gene ID (NCBI) 7284
RRIDAB_2880616
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP49411
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TUFM, also named as Elongation factor Tu, mitochondrial, is a 452 amino acid protein, which contains 1 tr-type G (guanine nucleotide-binding) domain and belongs to the TRAFAC class translation factor GTPase superfamily. TUFM localizes in the Mitochondrion and promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.

实验方案

Product Specific Protocols
WB protocol for TUFM antibody 26730-1-APDownload protocol
IF protocol for TUFM antibody 26730-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
zebrafishWB

Nucleic Acids Res

Deletion of Mtu1 (Trmu) in zebrafish revealed the essential role of tRNA modification in mitochondrial biogenesis and hearing function.

Authors - Qinghai Zhang
View Article
zebrafishWB

Nucleic Acids Res

Ablation of Mto1 in zebrafish exhibited hypertrophic cardiomyopathy manifested by mitochondrion RNA maturation deficiency.

Authors - Qinghai Zhang
View Article
ratWB

Sci Total Environ

Fluoride impairs mitochondrial translation by targeting miR-221-3p/c-Fos/RMND1 axis contributing to neurodevelopment defects

Authors - Dongjie Li
View Article
zebrafishWB

Nucleic Acids Res

Deletion of Gtpbp3 in zebrafish revealed the hypertrophic cardiomyopathy manifested by aberrant mitochondrial tRNA metabolism.

Authors - Danni Chen
View Article