验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells, U2OS cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
25856-1-AP targets TUBGCP2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag23066 Product name: Recombinant human TUBGCP2 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 553-902 aa of BC093770 Sequence: ALLELALRMSTANTDPFKDDLKIDLMPHDLITQLLRVLAIETKQEKAMAHADPTELALSGLEAFSFDYIVKWPLSLIINRKALTRYQMLFRHMFYCKHVERQLCSVWISNKTAKQHSLHSAQWFAGAFTLRQRMLNFVQNIQYYMMFEVMEPTWHILEKNLKSASNIDDVLGHHTGFLDTCLKDCMLTNPELLKVFSKLMSVCVMFTNCMQKFTQSMKLDGELGGQTLEHSTVLGLPAGAEERARKELARKHLAEHADTVQLVSGFEATINKFDKNFSAHLLDLLARLSIYSTSDCEHGMASVISRLDFNGFYTERLERLSAERSQKATPQVPVLRGPPAPAPRVAVTAQ 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | tubulin, gamma complex associated protein 2 |
| 别名 | GCP 2, GCP2, h103p, hGCP2, hGrip103, hSpc97, SPBC97, Spc97p, TUBGCP2 |
| 计算分子量 | 902 aa, 103 kDa |
| 观测分子量 | 103 kDa |
| GenBank蛋白编号 | BC093770 |
| 基因名称 | TUBGCP2 |
| Gene ID (NCBI) | 10844 |
| RRID | AB_2880271 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BSJ2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TUBGCP2 (tubulin-gamma-complex-associated protein 2) s TUBGCP2 is a core component of the g-TuRC nucleation complex (PMID: 3345861). Pathogenic variants in TUBGCP2 cause an autosomal recessive neurodevelopmental trait consisting of a neuronal migration disord (PMID: 31630790).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for TUBGCP2 antibody 25856-1-AP | Download protocol |
| IP protocol for TUBGCP2 antibody 25856-1-AP | Download protocol |
| WB protocol for TUBGCP2 antibody 25856-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
