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验证数据展示

  • WB analysis using 28009-1-AP

    WB analysis using 28009-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28009-1-AP (TUBG2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 28009-1-AP

    IHC staining of human lung cancer using 28009-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28009-1-AP (TUBG2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 28009-1-AP

    IF Staining of HepG2 using 28009-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TUBG2 antibody (28009-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of HepG2 using 28009-1-AP

    FC experiment of HepG2 using 28009-1-AP

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human TUBG2 (28009-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

TUBG2 Polyclonal antibody

TUBG2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 28009-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

tubulin, gamma 2, Tubulin gamma-2 chain, Tubulin gamma 2 chain, GTU-88, Gamma-2-tubulin

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse cerebellum tissue, HeLa cells, T-47D cells, rat cerebellum tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28009-1-AP targets TUBG2 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag27728

Product name: Recombinant human TUBG2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 358-451 aa of BC051890

Sequence: VALSRKSPYLPSAHRVSGLMMANHTSISSLFESSCQQFDKLRKRDAFLEQFRKEDMFKDNFDEMDRSREVVQELIDEYHAATQPDYISWGTQEQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tubulin, gamma 2
别名 tubulin, gamma 2, Tubulin gamma-2 chain, Tubulin gamma 2 chain, GTU-88, Gamma-2-tubulin
计算分子量 451 aa, 51 kDa
观测分子量51 kDa
GenBank蛋白编号BC051890
基因名称 TUBG2
Gene ID (NCBI) 27175
RRIDAB_2918138
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NRH3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for TUBG2 antibody 28009-1-APDownload protocol
IF protocol for TUBG2 antibody 28009-1-APDownload protocol
IHC protocol for TUBG2 antibody 28009-1-APDownload protocol
WB protocol for TUBG2 antibody 28009-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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