|Positive WB detected in||mouse cerebellum tissue, HeLa cells|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||mouse brain tissue, human brain tissue, rat brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||C2C12 cells, HepG2 cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:2000-1:10000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
10068-1-AP targets Beta Tubulin in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Applications||FC, IF, IHC, IP, WB, ELISA|
|Cited Applications||IF, IP, WB|
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, bovine, Caenorhabditis elegans, canine, mosue, pig, silkworm, zebrafish|
|Immunogen||Beta Tubulin fusion protein Ag0117|
|Host / Isotype||Rabbit / IgG|
|Full Name||tubulin, beta 3|
|Synonyms||beta 4, beta tubulin, B-tubulin, MC1R, TUBB, tubulin|
|Calculated molecular weight||450 aa, 50 kDa|
|Observed molecular weight||50-55 kDa|
|GenBank accession number||BC000748|
|Gene ID (NCBI)||10381|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart, once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha, beta, and gamma tubulins have all been used as loading controls. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs.
|Product Specific Protocols|
|WB protocol for Beta Tubulin antibody 10068-1-AP||Download protocol|
|IHC protocol for Beta Tubulin antibody 10068-1-AP||Download protocol|
|IF protocol for Beta Tubulin antibody 10068-1-AP||Download protocol|
|IP protocol for Beta Tubulin antibody 10068-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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