Beta Tubulin Polyclonal antibody

Beta Tubulin Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (7)

Applications

FC, IF, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 10068-1-AP

Print datasheet

Synonyms

beta 4, beta tubulin, B-tubulin, MC1R, TUBB, tubulin



经过测试的应用

Positive WB detected inmouse cerebellum tissue, HeLa cells
Positive IP detected inHEK-293 cells
Positive IHC detected inmouse brain tissue, human brain tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inC2C12 cells, HepG2 cells
Positive FC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:2000-1:10000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

产品信息

10068-1-AP targets Beta Tubulin in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IF, IHC, IP, WB, ELISA
Cited Applications IF, IP, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, bovine, Caenorhabditis elegans, canine, mosue, pig, silkworm, zebrafish
Immunogen Beta Tubulin fusion protein Ag0117 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tubulin, beta 3
Synonyms beta 4, beta tubulin, B-tubulin, MC1R, TUBB, tubulin
Calculated molecular weight 450 aa, 50 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC000748
Gene symbol TUBB3
UniprotQ13509
Gene ID (NCBI) 10381
RRIDAB_2303998
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart, once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha, beta, and gamma tubulins have all been used as loading controls. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs.

实验方案

Product Specific Protocols
WB protocol for Beta Tubulin antibody 10068-1-APDownload protocol
IHC protocol for Beta Tubulin antibody 10068-1-APDownload protocol
IF protocol for Beta Tubulin antibody 10068-1-APDownload protocol
IP protocol for Beta Tubulin antibody 10068-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell

Complement Signals Determine Opposite Effects of B Cells in Chemotherapy-Induced Immunity.

Authors - Yiwen Lu
mouseWB

Sci Adv

Loss of schizophrenia-related miR-501-3p in mice impairs sociability and memory by enhancing mGluR5-mediated glutamatergic transmission

Authors - Wenquan Liang
mouseWB

Sci Adv

PKM2 promotes pulmonary fibrosis by stabilizing TGF-β1 receptor I and enhancing TGF-β1 signaling

Authors - Shaoyan Gao
humanWB

Nat Commun

Identification of predictors of drug sensitivity using patient-derived models of esophageal squamous cell carcinoma.

Authors - Dan Su
humanWB

Nat Commun

Second messenger Ap4A polymerizes target protein HINT1 to transduce signals in FcεRI-activated mast cells.

Authors - Jing Yu
humanWB

Nucleic Acids Res

Activation of bivalent factor DLX5 cooperates with master regulator TP63 to promote squamous cell carcinoma.

Authors - Yongsheng Huang