Beta Tubulin Polyclonal antibody

Beta Tubulin Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (5)

Applications

FC, IF, IHC, IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 10094-1-AP

Print datasheet

Synonyms

beta tubulin, B-tubulin, tbb5, TUBB, β-tubulin



经过测试的应用

Positive WB detected inU-251 cells, HEK-293 cells, mouse kidney tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inrat brain tissue, mouse brain tissue, human colon tissue, human cerebellum tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite®488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

产品信息

10094-1-AP targets Beta Tubulin in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IF, IHC, IP, WB, ELISA
Cited Applications CoIP, IF, IHC, IP, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, bovine, fish, hamster, pig, zebrafish
Immunogen Beta Tubulin fusion protein Ag0136 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tubulin, beta 3
Synonyms beta tubulin, B-tubulin, tbb5, TUBB, β-tubulin
Calculated molecular weight 50 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC000748
Gene symbol TUBB3
UniprotQ13509
Gene ID (NCBI) 10381
RRIDAB_2210695
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart, once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha, beta, and gamma tubulins have all been used as loading controls. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs.

实验方案

Product Specific Protocols
WB protocol for Beta Tubulin antibody 10094-1-APDownload protocol
IHC protocol for Beta Tubulin antibody 10094-1-APDownload protocol
IF protocol for Beta Tubulin antibody 10094-1-APDownload protocol
IP protocol for Beta Tubulin antibody 10094-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nat Nanotechnol

Long-term pulmonary exposure to multi-walled carbon nanotubes promotes breast cancer metastatic cascades.

Authors - Xuefei Lu
mouseWB

Cell Metab

TMEM41B acts as an ER scramblase required for lipoprotein biogenesis and lipid homeostasis.

Authors - Dong Huang
mouseWB

Cell Metab

Ejection of damaged mitochondria and their removal by macrophages ensure efficient thermogenesis in brown adipose tissue.

Authors - Marco Rosina
humanWB

Nat Immunol

The tumor suppressor PTEN has a critical role in antiviral innate immunity.

Authors - Shun Li
humanIF

Adv Sci (Weinh)

The CRISPR-Cas13a Gene-Editing System Induces Collateral Cleavage of RNA in Glioma Cells.

Authors - Qixue Wang
mouseWB

Mol Cell

HRD1-mediated METTL14 degradation regulates m6A mRNA modification to suppress ER proteotoxic liver disease.

Authors - Juncheng Wei