Beta Tubulin Polyclonal antibody
Beta Tubulin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (7)
Applications
WB, IHC, IF/ICC, IF-P, IP, ELISA and More (2)
Conjugate
Unconjugated
905
验证数据展示
经过测试的应用
Positive WB detected in | U-251 cells, mouse kidney tissue, HEK-293 cells, rat kidney tissue, mouse brain tissue, rat brain tissue |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | human colon tissue, human cerebellum tissue, mouse brain tissue, rat testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse eye tissue |
Positive IF/ICC detected in | HeLa cells, HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:12000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
10094-1-AP targets Beta Tubulin in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF/ICC, IF-P, IP, ELISA Application Description |
Cited Applications | WB, IHC, IF, IP, CoIP |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, rabbit, chicken, zebrafish, hamster, sheep, goat, fish |
Immunogen | Beta Tubulin fusion protein Ag0136 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | tubulin, beta 3 |
Synonyms | Tubulin beta III, Tubulin beta 3 chain, Tubulin beta-III, Tubulin beta-4 chain, Tubulin beta-3 chain |
Calculated Molecular Weight | 50 kDa |
Observed Molecular Weight | 50-55 kDa |
GenBank Accession Number | BC000748 |
Gene Symbol | TUBB3 |
Gene ID (NCBI) | 10381 |
RRID | AB_2210695 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q13509 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for Beta Tubulin antibody 10094-1-AP | Download protocol |
IHC protocol for Beta Tubulin antibody 10094-1-AP | Download protocol |
IF protocol for Beta Tubulin antibody 10094-1-AP | Download protocol |
IP protocol for Beta Tubulin antibody 10094-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Biotechnol Magnify is a universal molecular anchoring strategy for expansion microscopy | ||
Nat Nanotechnol Long-term pulmonary exposure to multi-walled carbon nanotubes promotes breast cancer metastatic cascades. | ||
Cell Metab TMEM41B acts as an ER scramblase required for lipoprotein biogenesis and lipid homeostasis. | ||
Cell Metab Ejection of damaged mitochondria and their removal by macrophages ensure efficient thermogenesis in brown adipose tissue. | ||
Nat Cell Biol Single-cell multi-omics profiling of human preimplantation embryos identifies cytoskeletal defects during embryonic arrest | ||