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Alpha Tubulin Polyclonal antibody

Alpha Tubulin Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, zebrafish, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 16007-1-AP

Print datasheet

Synonyms

alpha tubulin, Alpha tubulin 6, TUBA1C, TUBA6, Tubulin alpha 1C chain, Tubulin alpha 6 chain, tubulin, alpha 1c

验证数据展示

  • WB analysis using 16007-1-AP

    WB analysis using 16007-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16007-1-AP (Alpha Tubulin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat eye using 16007-1-AP

    IHC staining of rat eye using 16007-1-AP

    Immunohistochemical analysis of paraffin-embedded rat eye tissue slide using 16007-1-AP (Alpha Tubulin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHeLa cells, NIH/3T3 cells, zebrafish tissues, mouse ovary tissues
Positive IHC detected inrat eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16007-1-AP targets Alpha Tubulin in WB, IHC, ELISA applications and shows reactivity with human, mouse, zebrafish, rat samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity human, mouse, zebrafish, rat
Immunogen Alpha Tubulin fusion protein Ag8695 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tubulin, alpha 1c
Synonyms alpha tubulin, Alpha tubulin 6, TUBA1C, TUBA6, Tubulin alpha 1C chain, Tubulin alpha 6 chain, tubulin, alpha 1c
Calculated Molecular Weight 50 kDa
Observed Molecular Weight50 kDa
GenBank Accession NumberBC004949
Gene Symbol TUBA1C
Gene ID (NCBI) 84790
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BQE3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Alpha Tubulin antibody 16007-1-APDownload protocol
IHC protocol for Alpha Tubulin antibody 16007-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols