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验证数据展示

  • WB analysis using 16007-1-AP

    WB analysis using 16007-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16007-1-AP (Alpha Tubulin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat eye using 16007-1-AP

    IHC staining of rat eye using 16007-1-AP

    Immunohistochemical analysis of paraffin-embedded rat eye tissue slide using 16007-1-AP (Alpha Tubulin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Alpha Tubulin Polyclonal antibody

Alpha Tubulin Polyclonal Antibody for WB, IHC, ELISA
Cat No. 16007-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, zebrafish, rat

应用

WB, IHC, ELISA

别名

alpha tubulin, Alpha tubulin 6, TUBA1C, TUBA6, Tubulin alpha 1C chain, Tubulin alpha 6 chain, tubulin, alpha 1c

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, NIH/3T3 cells, zebrafish tissues, mouse ovary tissues
Positive IHC detected inrat eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16007-1-AP targets Alpha Tubulin in WB, IHC, ELISA applications and shows reactivity with human, mouse, zebrafish, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, zebrafish, rat
免疫原

CatNo: Ag8695

Product name: Recombinant human tubulin-Alpha protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-449 aa of BC004949

Sequence: MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLTVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAVAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEEVGADSADGEDEGEEY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tubulin, alpha 1c
别名 alpha tubulin, Alpha tubulin 6, TUBA1C, TUBA6, Tubulin alpha 1C chain, Tubulin alpha 6 chain, tubulin, alpha 1c
计算分子量 50 kDa
观测分子量50 kDa
GenBank蛋白编号BC004949
基因名称 Alpha Tubulin
Gene ID (NCBI) 84790
RRIDAB_2303957
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BQE3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Alpha Tubulin antibody 16007-1-APDownload protocol
IHC protocol for Alpha Tubulin antibody 16007-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols