验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, mouse uterus tissue, Neuro-2a cells, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Neuro-2a cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17928-1-AP targets TUB in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, ChIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag12354 Product name: Recombinant human TUB protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 212-561 aa of BC075032 Sequence: GQSDHAQDAGETAAGGGERPSGQDLRATMQRKGISSSMSFDEDEEDEEENSSSSSQLNSNTRPSSATSRKSVREAASAPSPTAPEQPVDVEVQDLEEFALRPAPQGITIKCRITRDKKGMDRGMYPTYFLHLDREDGKKVFLLAGRKRKKSKTSNYLISVDPTDLSRGGDSYIGKLRSNLMGTKFTVYDNGVNPQKASSSTLESGTLRQELAAVCYETNVLGFKGPRKMSVIVPGMNMVHERVSIRPRNEHETLLARWQNKNTESIIELQNKTPVWNDDTQSYVLNFHGRVTQASVKNFQIIHGNDPDYIVMQFGRVAEDVFTMDYNYPLCALQAFAIALSSFDSKLACE 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | tubby homolog (mouse) |
| 别名 | rd5 |
| 计算分子量 | 561 aa, 62 kDa |
| 观测分子量 | 56 kDa, 62-67 kDa |
| GenBank蛋白编号 | BC075032 |
| 基因名称 | TUB |
| Gene ID (NCBI) | 7275 |
| RRID | AB_2212236 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P50607 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for TUB antibody 17928-1-AP | Download protocol |
| IHC protocol for TUB antibody 17928-1-AP | Download protocol |
| IP protocol for TUB antibody 17928-1-AP | Download protocol |
| WB protocol for TUB antibody 17928-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cells Differential Roles of Tubby Family Proteins in Ciliary Formation and Trafficking. | ||
Cell Rep NAD+ supplement potentiates tumor-killing function by rescuing defective TUB-mediated NAMPT transcription in tumor-infiltrated T cells.
| ||
Front Neurosci Distribution of ciliary adaptor proteins tubby and TULP3 in the organ of Corti | ||
Sci Transl Med The TUB variant impairs leptin sensitivity and AgRP neuronal response, leading to obesity | ||
Cell Commun Signal miR-10b-5p regulates adipocyte lineage commitment and adipogenesis via targeting of Gata6 and Tubby. | ||
