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验证数据展示

  • WB analysis using 26973-1-AP

    WB analysis using 26973-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26973-1-AP (TTYH1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human gliomas using 26973-1-AP

    IHC staining of human gliomas using 26973-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 26973-1-AP (TTYH1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human gliomas using 26973-1-AP

    IHC staining of human gliomas using 26973-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 26973-1-AP (TTYH1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TTYH1 Polyclonal antibody

TTYH1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 26973-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

hTTY1, Protein tweety homolog 1, TTYH1, tweety homolog 1 (Drosophila)

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman gliomas
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26973-1-AP targets TTYH1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 TTYH1 fusion protein Ag25290 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tweety homolog 1 (Drosophila)
别名 hTTY1, Protein tweety homolog 1, TTYH1, tweety homolog 1 (Drosophila)
计算分子量 49-50 kDa
观测分子量 50 kDa
GenBank蛋白编号BC011347
基因名称 TTYH1
Gene ID (NCBI) 57348
RRIDAB_2880707
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H313
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TTYH1, protein tweety homolog 1, is a glycosylated protein composed of five predicted transmembrane segments, which belongs to the tweety family. The expression of TTYH1 is restricted mainly to neural tissue and is up-regulated in astrocytoma, glioma and several other cancers. TTYH1 has some isoforms with MW of 49-50 kDa, 35 kDa and 26 kDa.

实验方案

Product Specific Protocols
WB protocol for TTYH1 antibody 26973-1-APDownload protocol
IHC protocol for TTYH1 antibody 26973-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

J Cancer

Elevated expression of Tweety homologue 3 predicts poor clinical outcomes in ovarian cancer.

Authors - Junhan Zhou
View Article