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TTRAP Polyclonal antibody

TTRAP Polyclonal Antibody for WB, IP, ELISA
Cat No. 12203-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, ELISA

EAP2, AD-022, AD022, 5'-tyrosyl-DNA phosphodiesterase, 5'-Tyr-DNA phosphodiesterase

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存


经过测试的应用

Positive WB detected inHEK-293T cells, HepG2 cells, human spleen tissue, HeLa cells
Positive IP detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy TDP2 Ready-To-Use IHC Kit. TDP2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12203-1-AP targets TTRAP in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原

CatNo: Ag2846

Product name: Recombinant human TTRAP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-362 aa of BC017553

Sequence: MELGSCLEGGREAAEEEGEPEVKKRRLLCVEFASVASCDAAVAQCFLAENDWEMERALNSYFEPPVEESALERRPETISEPKTYVDLTNEETTDSTTSKISPSEDTQQENGSMFSLITWNIDGLDLNNLSERARGVCSYLALYSPDVIFLQEVIPPYYSYLKKRSSNYEIITGHEEGYFTAIMLKKSRVKLKSQEIIPFPSTKMMRNLLCVHVNVSGNELCLMTSHLESTRGHAAERMNQLKMVLKKMQEAPESATVIFAGDTNLRDREVTRCGGLPNNIVDVWEFLGKPKHCQYTWDTQMNSNLGITAACKLRFDRIFFRAAAEEGHIIPRSLDLLGLEKLDCGRFPSDHWGLLCNLDIIL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 TRAF and TNF receptor associated protein
别名 EAP2, AD-022, AD022, 5'-tyrosyl-DNA phosphodiesterase, 5'-Tyr-DNA phosphodiesterase
计算分子量 362 aa, 41 kDa
观测分子量 49 kDa
GenBank蛋白编号BC017553
基因名称 TTRAP
Gene ID (NCBI) 51567
RRIDAB_1607504
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95551
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TTRAP is a 5-prime tyrosyl-DNA phosphodiesterase that is required for efficient repair of topoisomerase II-induced DNA double-strand breaks. Thus it can remove lots of covalent adducts from DNA through hydrolysis of a 5'-phosphodiester bond, generating a free 5' phosphate to DNA. It also has 3'-tyrosyl DNA phophodiesterase activity, but not so efficient as TDP1. TTRAP functions as a negative regulator of ETS1 and inhibit nuclear factor-kappa-B activation.

实验方案

Product Specific Protocols
WB protocol for TTRAP antibody 12203-1-APDownload protocol
IP protocol for TTRAP antibody 12203-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nucleic Acids Res

Reversal of DNA damage induced Topoisomerase 2 DNA-protein crosslinks by Tdp2.

Authors - Matthew J Schellenberg
mouseWB

Cell Death Differ

NBS1 is required for SPO11-linked DNA double-strand break repair in male meiosis.

Authors - Bin Zhang
  • KO Validated
humanWB

Anal Biochem

Generation of assays and antibodies to facilitate the study of human 5'-tyrosyl DNA phosphodiesterase.

Authors - Graeme Thomson
humanWB

Int J Mol Sci

Combined PARP and Dual Topoisomerase Inhibition Potentiates Genome Instability and Cell Death in Ovarian Cancer

Authors - Inken Flörkemeier
mouseWB

bioRxiv

DNA polymerase theta-mediated DNA repair is a functional dependency and therapeutic vulnerability in DNMT3A deficient leukemia cells

Authors - Bac Viet Le
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