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验证数据展示

  • WB analysis using 84493-6-RR

    WB analysis using 84493-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84493-6-RR (TTLL12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84493-6-RR

    Affinity and Kinetic Characterization of 84493-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84493-6-RR against Human TTLL12 were performed. The affinity constant is 57.3 pM.

TTLL12 Recombinant monoclonal antibody

TTLL12 Recombinant Antibody for WB, ELISA
Cat No. 84493-6-RR

产品说明书

CloneNo. 241904F4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

Tubulin--tyrosine ligase-like protein 12, Inactive tubulin--tyrosine ligase-like protein 12, 241904F4

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, SKOV-3 cells, PC-3 cells, HeLa cells, K-562 cells, DU 145 cells
Planning an IHC experiment? We recommend our IHCeasy TTLL12 Ready-To-Use IHC Kit. TTLL12 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84493-6-RR targets TTLL12 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag13804

Product name: Recombinant human TTLL12 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 293-644 aa of BC001070

Sequence: DINPVVHPHGHIFKVYTDVQQVASSLTHPRFTLTQSEADADILFNFSHFKDYRKLSQERPGVLLNQFPCENLLTVKDCLASIARRAGGPEGPPWLPRTFNLRTELPQFVSYFQQRERWGEDNHWICKPWNLARSLDTHVTKSLHSIIRHRESTPKVVSKYIESPVLFLREDVGKVKFDIRYIVLLRSVRPLRLFVYDVFWLRFSNRAFALNDLDDYEKHFTVMNYDPDVVLKQVHCEEFIPEFEKQYPEFPWTDVQAEIFRAFTELFQVACAKPPPLGLCDYPSSRAMYAVDLMLKWDNGPDGRRVMQPQILEVNFNPDCERACRYHPTFFNDVFSTLFLDQPGGCHVTCLV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 tubulin tyrosine ligase-like family, member 12
别名 Tubulin--tyrosine ligase-like protein 12, Inactive tubulin--tyrosine ligase-like protein 12, 241904F4
计算分子量 644 aa, 74 kDa
观测分子量 75 kDa
GenBank蛋白编号BC001070
基因名称 TTLL12
Gene ID (NCBI) 23170
RRIDAB_3672006
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ14166
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TTLL12 is a member of the tubulin tyrosine ligase (TTL) family that is highly conserved in phylogeny. TTLL12 contains 644 amino acids and has a SET-like domain and a TTL-like domain. TTLL12 is involved in the regulation of tubulin and histone modification and is a negative regulator of RNA virus-induced type I interferon. During the cell cycle, Both trimethylation of H4K20 and tubulin detyrosination levels were altered by TTLL2 expression (PMID: 23251473).

实验方案

Product Specific Protocols
WB protocol for TTLL12 antibody 84493-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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