验证数据展示
经过测试的应用
| Positive WB detected in | Hela cell |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
60235-1-Ig targets TTF1-Specific in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | transcription termination factor, RNA polymerase I |
| 别名 | transcription termination factor-Specific, TTF1, 7B3H2, TTF 1, TTF I |
| 计算分子量 | 103 kDa |
| 观测分子量 | 106 kDa |
| GenBank蛋白编号 | NM_007344 |
| 基因名称 | TTF1 |
| Gene ID (NCBI) | 7270 |
| RRID | AB_2881359 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q15361 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TTF1 is a multifunctional nucleolar protein that terminates ribosomal gene transcription, mediates replication fork arrest and regulates RNA polymerase I transcription on chromatin. It plays a dual role in rDNA regulation, being involved in both activation and silencing of rDNA transcription. Interaction with BAZ2A/TIP5, TTF1 recovers DNA-binding activity. The antibody is specific to TTF1.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TTF1-Specific antibody 60235-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
