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验证数据展示

  • IF Staining of hTERT-RPE1 using 31797-1-AP

    IF Staining of hTERT-RPE1 using 31797-1-AP

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using TTC9C antibody (31797-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CoraLite®594 ARL13B antibody (CL594-17711, red).

  • WB analysis using 31797-1-AP

    WB analysis using 31797-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31797-1-AP (TTC9C antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 31797-1-AP

    IHC staining of mouse testis using 31797-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 31797-1-AP (TTC9C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse testis using 31797-1-AP

    IF Staining of mouse testis using 31797-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using TTC9C antibody (31797-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TTC9C Polyclonal antibody

TTC9C Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 31797-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IF-P, ELISA

别名

TPR repeat protein 9C, Tetratricopeptide repeat protein 9C

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inTHP-1 cells, HeLa cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse testis tissue
Positive IF/ICC detected inhTERT-RPE1 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31797-1-AP targets TTC9C in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IF-P, ELISA Application Description
经测试反应性 human, mouse
免疫原 TTC9C fusion protein Ag37081 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tetratricopeptide repeat domain 9C
别名 TPR repeat protein 9C, Tetratricopeptide repeat protein 9C
观测分子量22 kDa
GenBank蛋白编号BC032123
基因名称 TTC9C
Gene ID (NCBI) 283237
RRIDAB_3670117
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ8N5M4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TTC9 family belongs to tetratricopeptide repeat (TPR)-containing proteins family (PMID: 35949301).The TPR motifs are arranged in antiparallel α-helical hairpins which are clustered to form flexible grooved interfaces. Members in TRP-containing proteins family are involved in a diverse range of biological functions, including cell cycle control, transcription, protein folding, and steroid receptor signaling (PMID: 15497498;PMID: 10786835).

实验方案

Product Specific Protocols
WB protocol for TTC9C antibody 31797-1-APDownload protocol
IHC protocol for TTC9C antibody 31797-1-APDownload protocol
IF protocol for TTC9C antibody 31797-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols