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验证数据展示

  • WB analysis of mouse testis using 12505-1-AP

    WB analysis of mouse testis using 12505-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 12505-1-AP (BBS8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 12505-1-AP

    IP experiment of HeLa using 12505-1-AP

    IP result of anti-BBS8 (IP:12505-1-AP, 3ug; Detection:12505-1-AP 1:400) with HeLa cells lysate 2000ug.

BBS8 Polyclonal antibody

BBS8 Polyclonal Antibody for WB, IP, ELISA
Cat No. 12505-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, ELISA

别名

BBS8, TPR repeat protein 8, TTC8

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse testis tissue, human lung tissue, NIH/3T3 cells
Positive IP detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12505-1-AP targets BBS8 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 BBS8 fusion protein Ag3186 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tetratricopeptide repeat domain 8
别名 BBS8, TPR repeat protein 8, TTC8
计算分子量 531 aa, 60 kDa
观测分子量 50-52 kDa
GenBank蛋白编号BC026351
基因名称 BBS8
Gene ID (NCBI) 123016
RRIDAB_2211953
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TAM2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BBS8 (also known as TTC8) is a subunit of BBSome complex which is necessary for ciliary membrane biogenesis. BBS8 localizes specifically to ciliated structures, such as the connecting cilium of the retina and columnar epithelial cells in the lung. Defects in BBS8 cause Bardet-Biedl syndrome type 8 (BBS8), an genetic disorder characterised by retinal degeneration, obesity, polydactyly, cognitive impairment and kidney failure. BBS8 generates two alternatively spliced isoforms. Western blot analysis using this antibody detected a major band around 60 kD in lung tissue.

实验方案

Product Specific Protocols
WB protocol for BBS8 antibody 12505-1-APDownload protocol
IP protocol for BBS8 antibody 12505-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols