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TTC5 Polyclonal antibody

TTC5 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 26112-1-AP

Print datasheet

Synonyms

Strap, TPR repeat protein 5, TTC5

验证数据展示

  • WB analysis of Jurkat using 26112-1-AP

    WB analysis of Jurkat using 26112-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 26112-1-AP (TTC5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 26112-1-AP

    IHC staining of human colon cancer using 26112-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26112-1-AP (TTC5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TTC5 antibody included
  • IHC staining of human colon cancer using 26112-1-AP

    IHC staining of human colon cancer using 26112-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26112-1-AP (TTC5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TTC5 antibody included

经过测试的应用

Positive WB detected inJurkat cells, RAW 264.7 cells
Positive IHC detected inhuman colon cancer tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy TTC5 Ready-To-Use IHC Kit. TTC5 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26112-1-AP targets TTC5 in WB, IHC, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity human, rat, mouse
Immunogen TTC5 fusion protein Ag23523 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tetratricopeptide repeat domain 5
Synonyms Strap, TPR repeat protein 5, TTC5
Observed Molecular Weight 45 kDa~49 kDa
GenBank Accession NumberBC053538
Gene Symbol TTC5
Gene ID (NCBI) 91875
RRIDAB_2880384
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N0Z6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TTC5 antibody 26112-1-APDownload protocol
IHC protocol for TTC5 antibody 26112-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols