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验证数据展示

  • WB analysis of HeLa using 11878-1-AP

    WB analysis of HeLa using 11878-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11878-1-AP (TTC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human lymphoma using 11878-1-AP

    IHC staining of human lymphoma using 11878-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 11878-1-AP (TTC4 antibody) at dilution of 1:50 (under 10x lens).

  • WB analysis of HEK-293 using 11878-1-AP

    WB analysis of HEK-293 using 11878-1-AP

    WB result of TTC4 antibody (11878-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TTC4 transfected HEK-293 cells.

TTC4 Polyclonal antibody

TTC4 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11878-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

TPR repeat protein 4, TTC4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11878-1-AP targets TTC4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag2474

Product name: Recombinant human TTC4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-387 aa of BC001276

Sequence: MEQPGQDPTSDDVMDSFLEKFQSQPYRGGFHEDQWEKEFEKVPLFMTRAPSEIDPRENPDLACLQSIIFDEERSPEEQAKTYKDEGNDYFKEKDYKKAVISYTEGLKKKCADPDLNAVLYTNRAAAQYYLGNFRSALNDVTAARKLKPCHLKAIIRGALCHLELKHFAEAVNWCDEGLQIDAKEKKLLEMRAKADKLKRIEQRDVRKANLKEKKERNQNEALLQAIKARNIRLSEAACEDEDSASEGLGELFLDGLSTENPHGARLSLDGQGRLSWPVLFLYPEYAQSDFISAFHEDSRFIDHLMVMFGETPSWDLEQKYCPDNLEVYFEDEDRAELYRVPAKSTLLQVLQHQRYFVKALTPAFLVCVGSSPFCKNFLRGRKVYQIR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tetratricopeptide repeat domain 4
别名 TPR repeat protein 4, TTC4
计算分子量 387 aa, 45 kDa
观测分子量 45 kDa
GenBank蛋白编号BC001276
基因名称 TTC4
Gene ID (NCBI) 7268
RRIDAB_2211700
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95801
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TTC4 antibody 11878-1-APDownload protocol
IHC protocol for TTC4 antibody 11878-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols