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验证数据展示

  • WB analysis of PC-3 using 21693-1-AP

    WB analysis of PC-3 using 21693-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 21693-1-AP (TTC31 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 21693-1-AP

    IHC staining of human testis using 21693-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 21693-1-AP (TTC31 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human testis using 21693-1-AP

    IHC staining of human testis using 21693-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 21693-1-AP (TTC31 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of PC-3 using 21693-1-AP

    IF Staining of PC-3 using 21693-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed PC-3 cells using 21693-1-AP (TTC31 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

TTC31 Polyclonal antibody

TTC31 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 21693-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

TPR repeat protein 31

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inPC-3 cells
Positive IHC detected inhuman testis tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21693-1-AP targets TTC31 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag16281

Product name: Recombinant human TTC31 protein

Source: e coli.-derived, PKG

Tag: GST

Domain: 1-358 aa of BC098365

Sequence: MAPIPKTVGRIKLDCSLRPSCPLEVAAAPKLCKEFGPEDYGEEDIVDFLRRLVESDPQGLHRIHVDGSSGRLQLWHHDYLLGHLDDEGKSTGQSDRGKGAEGLGTYCGLRKSFLYPPQESEPCPQSPSASATFPSVSDSLLQVAMPQKLLVTEEEANRLAEELVAEEERMKQKAEKKRLKKKRQKERKRQERLEQYCGEPKASTTSDGDESPPSSPGNPVQGQCGEEEDSLDLSSTFVSLALRKVGDWPLSARREKGLNQEPQGRGLALQKMGQEEESPPREERPQQSPKEKDLGRLRPQDLLDFAPYPQASPGLLAAALQQSQELAKLGTSFAQNGFYHEAVVLFTQALKLNPQDHR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tetratricopeptide repeat domain 31
别名 TPR repeat protein 31
计算分子量 358 aa, 40 kDa
观测分子量 57 kDa
GenBank蛋白编号BC098365
基因名称 TTC31
Gene ID (NCBI) 64427
RRIDAB_11182824
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ49AM3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for TTC31 antibody 21693-1-APDownload protocol
IHC protocol for TTC31 antibody 21693-1-APDownload protocol
WB protocol for TTC31 antibody 21693-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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