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验证数据展示

  • WB analysis using 25352-1-AP

    WB analysis using 25352-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25352-1-AP (TTC30A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of A431 using 25352-1-AP

    WB analysis of A431 using 25352-1-AP

    WB result of TTC30A antibody (25352-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TTC30A transfected A431 cells.

  • IF Staining of hTERT-RPE1 using 25352-1-AP

    IF Staining of hTERT-RPE1 using 25352-1-AP

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using TTC30A antibody (25352-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), acetylated Tubulin(Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red).

  • IP experiment of HEK-293 using 25352-1-AP

    IP experiment of HEK-293 using 25352-1-AP

    IP result of anti-TTC30A (IP:25352-1-AP, 4ug; Detection:25352-1-AP 1:500) with HEK-293 cells lysate 2600ug.

  • Featured Product
  • KD/KO验证

TTC30A Polyclonal antibody

TTC30A Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 25352-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, IP, ELISA and More (1)

别名

IFT70A, TPR repeat protein 30A, Intraflagellar transport protein 70A

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, HEK-293 cells, mouse testis, rat testis
Positive IP detected inHEK-293 cells
Positive IF/ICC detected inhTERT-RPE1 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25352-1-AP targets TTC30A in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 TTC30A fusion protein Ag18361 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tetratricopeptide repeat domain 30A
别名 IFT70A, TPR repeat protein 30A, Intraflagellar transport protein 70A
计算分子量 665 aa, 76 kDa
观测分子量 72-76 kDa
GenBank蛋白编号BC042848
基因名称 TTC30A
Gene ID (NCBI) 92104
RRIDAB_2651172
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86WT1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TTC30A antibody 25352-1-APDownload protocol
IF protocol for TTC30A antibody 25352-1-APDownload protocol
IP protocol for TTC30A antibody 25352-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Dev Cell

The CEP19-RABL2 GTPase Complex Binds IFT-B to Initiate Intraflagellar Transport at the Ciliary Base.

Authors - Tomoharu Kanie
View Article
humanWB,IF

Biol Open

Robust interaction of IFT70 with IFT52-IFT88 in the IFT-B complex is required for ciliogenesis.

Authors - Ryota Takei
View Article