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验证数据展示

  • IHC staining of mouse brain using 15072-1-AP (same clone as 15072-1-PBS)

    IHC staining of mouse brain using 15072-1-AP (same clone as 15072-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15072-1-AP (TTBK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15072-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TTBK2 antibody included
  • IHC staining of mouse brain using 15072-1-AP (same clone as 15072-1-PBS)

    IHC staining of mouse brain using 15072-1-AP (same clone as 15072-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15072-1-AP (TTBK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15072-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TTBK2 antibody included
  • WB analysis of PC-3 using 15072-1-AP (same clone as 15072-1-PBS)

    WB analysis of PC-3 using 15072-1-AP (same clone as 15072-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 15072-1-AP (TTBK2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15072-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 15072-1-AP (same clone as 15072-1-PBS)

    WB analysis of HeLa using 15072-1-AP (same clone as 15072-1-PBS)

    WB result of TTBK2 antibody (15072-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TTBK2 transfected HeLa cells. This data was developed using the same antibody clone with 15072-1-PBS in a different storage buffer formulation.

  • IF Staining of hTERT-RPE1 using 15072-1-AP (same clone as 15072-1-PBS)

    IF Staining of hTERT-RPE1 using 15072-1-AP (same clone as 15072-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using TTBK2 antibody (15072-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 ARL13B antibody (CL594-17711, red). This data was developed using the same antibody clone with 15072-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

TTBK2 Polyclonal antibody, PBS Only

TTBK2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 15072-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy TTBK2 Ready-To-Use IHC Kit. TTBK2 primary antibody included.

产品信息

15072-1-PBS targets TTBK2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag7108

Product name: Recombinant human TTBK2 protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 1-354 aa of BC071556

Sequence: MSGGGEQPDILSVGILVKERWKVLRKIGGGGFGEIYDALDMLTRENVALKVESAQQPKQVLKMEVAVLKKLQGKDHVCRFIGCGRNDRFNYVVMQLQGRNLADLRRSQSRGTFTISTTLRLGRQILESIESIHSVGFLHRDIKPSNFAMGRFPSTCRKCYMLDFGLARQFTNSCGDVRPPRAVAGFRGTVRYASINAHRNREMGRHDDLWSLFYMLVEFVVGQLPWRKIKDKEQVGSIKERYDHRLMLKHLPPEFSIFLDHISSLDYFTKPDYQLLTSVFDNSIKTFGVIESDPFDWEKTGNDGSLTTTTTSTTPQLHTRLTPAAIGIANATPIPGDLLRENTDEVFPDEQLSD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tau tubulin kinase 2
别名
计算分子量 137 kDa
观测分子量 137-150 kDa
GenBank蛋白编号BC071556
基因名称 TTBK2
Gene ID (NCBI) 146057
RRIDAB_2211507
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6IQ55
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TTBK2(tau tubulin kinase 2) plays an important role in the tau cascade and in spinocerebellar degeneration. The N-terminus of TTBK2 has a serine-threonine-tyrosine kinase domain, and a C-terminal region shows homology to TTBK1. TTBK1 and TTBK2 function as regulators of TDP-43 phosphorylation. TTBK1/2 may be attractive drug targets for therapeutic interventions in TDP-43 proteinopathies such as FTLD-TDP and ALS.(PMID:25473830). 15072-1-AP has a cross reaction with TTBK1.

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