验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HEK-293 cells, human brain tissue, mouse brain tissue, mouse liver tissue, rat liver tissue |
| Positive IHC detected in | rat kidney tissue, human kidney tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:8000-1:32000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 7 publications below |
| IHC | See 1 publications below |
产品信息
66018-1-Ig targets TST in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag9235 Product name: Recombinant human TST protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-297 aa of BC010148 Sequence: MVHQVLYRALVSTKWLAESIRTGKLGPGLRVLDASWYSPGTREARKEYLERHVPGASFFDIEECRDTASPYEMMLPSEAGFAEYVGRLGISNHTHVVVYDGEHLGSFYAPRVWWMFRVFGHRTVSVLNGGFRNWLKEGHPVTSEPSRPEPAVFKATLDRSLLKTYEQVLENLESKRFQLVDSRSQGRFLGTEPEPDAVGLDSGHIRGAVNMPFMDFLTEDGFEKGPEELRALFQTKKVDLSQPLIATCRKGVTACHVALAAYLCGKPDVAVYDGSWSEWFRRAPPESRVSQGKSEKA 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | thiosulfate sulfurtransferase (rhodanese) |
| 别名 | 2A6C5, EC:2.8.1.1, RDS, Rhodanese |
| 计算分子量 | 297 aa, 33 kDa |
| 观测分子量 | 33 kDa |
| GenBank蛋白编号 | BC010148 |
| 基因名称 | TST |
| Gene ID (NCBI) | 7263 |
| RRID | AB_11045660 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q16762 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Thiosulfate sulfurtransferase (TST), also known as rhodanese, is a mitochondrial enzyme involved in cyanide detoxification and the formation of iron-sulfur proteins and the modification of sulfur-containing enzymes.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for TST antibody 66018-1-Ig | Download protocol |
| IHC protocol for TST antibody 66018-1-Ig | Download protocol |
| WB protocol for TST antibody 66018-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Biol Chem Polymorphic variants of human rhodanese exhibit differences in thermal stability and sulfurtransfer kinetics. | ||
Biochem Pharmacol Drug resistance induces the upregulation of H2S-producing enzymes in HCT116 colon cancer cells. | ||
J Biol Chem Hydrogen sulfide perturbs mitochondrial bioenergetics and triggers metabolic reprogramming in colon cells. | ||
Biomolecules Sulfurtransferases and Cystathionine Beta-Synthase Expression in Different Human Leukemia Cell Lines. | ||
Mol Biol Rep Curcumin reduces myocardial ischemia-reperfusion injury, by increasing endogenous H2S levels and further modulating m6A | ||
Burns Cystathionine-gamma-lyase deficient mice are protected against the development of multiorgan failure and exhibit reduced inflammatory response during burn. |
