验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13806-1-AP targets TSSK2 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | TSSK2 fusion protein Ag4805 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | testis-specific serine kinase 2 |
| 别名 | Serine/threonine-protein kinase 22B, EC:2.7.11.1, DiGeorge syndrome protein G, DGS-G, DGSG |
| 计算分子量 | 358 aa, 41 kDa |
| 观测分子量 | 40 kDa |
| GenBank蛋白编号 | BC037781 |
| 基因名称 | TSSK2 |
| Gene ID (NCBI) | 23617 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96PF2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TSSK2 (Testis-Specific Serine/Threonine Kinase 2) is a member of the TSSK family, predominantly expressed in the testis and involved in spermiogenesis and male fertility. It is localized in various regions of sperm, including the centrioles of post-meiotic spermatids, the tail, acrosomal regions, and the midpiece. TSSK2 plays a crucial role in sperm maturation by phosphorylating substrates such as TSKS and SPAG16L. Studies have shown that TSSK2 knockout mice exhibit infertility due to impaired spermatid elongation and sperm maturation. Additionally, TSSK2 is considered a promising target for reversible male contraception, with several potent inhibitors identified through high-throughput screening.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TSSK2 antibody 13806-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |