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验证数据展示

  • IHC staining of human lung using 14531-1-AP

    IHC staining of human lung using 14531-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 14531-1-AP (TSSC4 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human lung using 14531-1-AP

    IHC staining of human lung using 14531-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 14531-1-AP (TSSC4 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HeLa using 14531-1-AP

    IF Staining of HeLa using 14531-1-AP

    Immunofluorescent analysis of Hela cells, using TSSC4 antibody 14531-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • IHC staining of mouse lung using 14531-1-AP

    IHC staining of mouse lung using 14531-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 14531-1-AP (TSSC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

TSSC4 Polyclonal antibody

TSSC4 Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 14531-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF/ICC, ELISA and More (1)

别名

U5 small nuclear ribonucleoprotein TSSC4, Tumor-suppressing subchromosomal transferable fragment candidate gene 4 protein, Tumor-suppressing STF cDNA 4 protein, Protein TSSC4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inhuman lung tissue, mouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14531-1-AP targets TSSC4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human
免疫原 TSSC4 fusion protein Ag6030 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tumor suppressing subtransferable candidate 4
别名 U5 small nuclear ribonucleoprotein TSSC4, Tumor-suppressing subchromosomal transferable fragment candidate gene 4 protein, Tumor-suppressing STF cDNA 4 protein, Protein TSSC4
计算分子量 34 kDa
观测分子量35-45 kDa
GenBank蛋白编号BC050616
基因名称 TSSC4
Gene ID (NCBI) 10078
RRIDAB_11232415
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y5U2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for TSSC4 antibody 14531-1-APDownload protocol
IF protocol for TSSC4 antibody 14531-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Commun

TSSC4 is a component of U5 snRNP that promotes tri-snRNP formation.

Authors - Klára Klimešová
  • KD Validated
View Article
humanWB

Front Immunol

Alternative splicing regulation by tumor suppressing subtransferable candidate 4: a pathway to tumor suppression

Authors - Haiping Zhao
View Article