验证数据展示
经过测试的应用
| Positive IHC detected in | human lung tissue, mouse lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Hela cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
产品信息
14531-1-AP targets TSSC4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag6030 Product name: Recombinant human TSSC4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-265 aa of BC050616 Sequence: MAEAGTGLLPATVQPFHLRGMSSTFSQRSRDIFDCLEGAARRAPSSVAHTSMSDNGGFKRPLAPSGRSPVEGLGRAHRSPASPRVPPVPDYVAHPERWTKYSLEDVTEVSEQSNQATALAFLGSQSLAAPTDCVSSFNQDPSSCGEGRVIFTKPVRGVEARHERKRVLGKVGEPGRGGLGNPATDRGEGPVELAHLAGPGSPEAEEWGSPHGGLQEVEALSGSVHSGSVPGLPPVETVGFHGSRKRSRDHFRNKSSSPEDPGAEV 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | tumor suppressing subtransferable candidate 4 |
| 别名 | U5 small nuclear ribonucleoprotein TSSC4, Tumor-suppressing subchromosomal transferable fragment candidate gene 4 protein, Tumor-suppressing STF cDNA 4 protein, Protein TSSC4 |
| 计算分子量 | 34 kDa |
| 观测分子量 | 35-45 kDa |
| GenBank蛋白编号 | BC050616 |
| 基因名称 | TSSC4 |
| Gene ID (NCBI) | 10078 |
| RRID | AB_11232415 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y5U2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for TSSC4 antibody 14531-1-AP | Download protocol |
| IHC protocol for TSSC4 antibody 14531-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun TSSC4 is a component of U5 snRNP that promotes tri-snRNP formation.
| ||
Front Immunol Alternative splicing regulation by tumor suppressing subtransferable candidate 4: a pathway to tumor suppression |
