验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HepG2 cells, HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
19852-1-AP targets TSSC1 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag13923 Product name: Recombinant human TSSC1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-387 aa of BC002485 Sequence: MEDDAPVIYGLEFQARALTPQTAETDAIRFLVGTQSLKYDNQIHIIDFDDENNIINKNVLLHQAGEIWHISASPADRGVLTTCYNRTSDSKVLTCAAVWRMPKELESGSHESPDDSSSTAQTLELLCHLDNTAHGNMACVVWEPMGDGKKIISLADNHILLWDLQESSSQAVLASSASLEGKGQLKFTSGRWSPHHNCTQVATANDTTLRGWDTRSMSQIYCIENAHGQLVRDLDFNPNKQYYLASCGDDCKVKFWDTRNVTEPVKTLEEHSHWVWNVRYNHSHDQLVLTGSSDSRVILSNMVSISSEPFGHLVDDDDISDQEDHRSEEKSKEPLQDNVIATYEEHEDSVYAVDWSSADPWLFASLSYDGRLVINRVPRALKYHILL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | tumor suppressing subtransferable candidate 1 |
| 别名 | EARP and GARP complex-interacting protein 1, EIPR1, Endosome-associated recycling protein-interacting protein, Golgi-associated retrograde protein-interacting protein, Protein TSSC1 |
| 计算分子量 | 387 aa, 44 kDa |
| 观测分子量 | ~50 kDa |
| GenBank蛋白编号 | BC002485 |
| 基因名称 | TSSC1 |
| Gene ID (NCBI) | 7260 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q53HC9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TSSC1 (Tumor Suppressing Subtransferable Candidate 1), now officially designated EIPR1, encodes a 387-amino-acid WD40 repeat protein that localizes to chromosome 2p25.3 in humans. Although originally identified within a putative tumor-suppressor region at 11p15.5, later genomic mapping reassigned the gene to chromosome 2. Functionally, TSSC1 is a component of the endosomal retrieval machinery, interacting with both the EARP and GARP tethering complexes to regulate protein transport from early endosomes to the trans-Golgi network and recycling endosomes. Gershlick et al. demonstrated that TSSC1 is required for efficient recruitment of GARP to the trans-Golgi network and functions as a regulator of both GARP and EARP in endosomal retrieval pathways (PMID: 27440922).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TSSC1 antibody 19852-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
