验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, Jurkat cells, MCF-7 cells, MDA-MB-231 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
27617-1-AP targets TSPYL5 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag26314 Product name: Recombinant human TSPYL5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-210 aa of BC045630 Sequence: MSGRSRGRKSSRAKNRGKGRAKARVRPAPDDAPRDPDPSQYQSLGEDTQAAQVQAGAGWGGLEAAASAQLLRLGEEAACRLPLDCGLALRARAAGDHGQAAARPGPGKAASLSERLAADTVFVGTAGTVGRPKNAPRVGNRRGPAGKKAPETCSTAGRGPQVIAGGRQKKGAAGENTSVSAGEEKKEERDAGSGPPATEGSMDTLENVQL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | TSPY-like 5 |
| 别名 | KIAA1750, TSPY like 5, TSPY like protein 5 |
| 计算分子量 | 417 aa, 45 kDa |
| 观测分子量 | 45-50 kDa |
| GenBank蛋白编号 | BC045630 |
| 基因名称 | TSPYL5 |
| Gene ID (NCBI) | 85453 |
| RRID | AB_3742244 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q86VY4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TSPYL5 (Testis-specific Y-encoded-like protein 5) is involved in modulation of cell growth and cellular response to gamma radiation probably via regulation of the Akt signaling pathway. It is also involved in the regulation of p53/TP53, and suppresses p53/TP53 protein levels and promotes its ubiquitination; Its function is dependent on USP7 and independent on MDM2 (PMID: 20079711; 21170034).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TSPYL5 antibody 27617-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
