TSPAN9 Polyclonal antibody

TSPAN9 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 21983-1-AP

Print datasheet

Synonyms

NET 5, NET5, PP1057, Tetraspan NET 5, tetraspanin 9, Tspan 9, TSPAN9



经过测试的应用

Positive WB detected inmouse peripheral blood leukocyte, mouse spleen tissue, rat spleen tissue
Positive IP detected inmouse spleen tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 21983-1-AP is TSPAN9 Fusion Protein expressed in E. coli.

Tested Applications IP, WB, ELISA
Cited ApplicationsWB, FC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen TSPAN9 fusion protein Ag17048 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tetraspanin 9
Synonyms NET 5, NET5, PP1057, Tetraspan NET 5, tetraspanin 9, Tspan 9, TSPAN9
Calculated Molecular Weight 239 aa, 27 kDa
Observed Molecular Weight 29-33 kDa
GenBank Accession NumberBC071881
Gene Symbol TSPAN9
Gene ID (NCBI) 10867
RRIDAB_2878960
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75954
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TSPAN9 is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Tetraspanins have been involved in diverse processes such as cell activation and proliferation, adhesion and motility, differentiation, and cancer. TSPAN9 is relatively highly expressed in the megakaryocyte/platelet lineage and has a role in regulating platelet function in concert with other platelet tetraspanins and their associated proteins. TSPAN9 has been identified as a glycoprotein (PMID: 18795891). This polyclonal antibody detects endogenous TSPAN9 at a band of 29-33 kDa, which is larger than the calculated molecular weight of TSPAN9 (27 kDa), probably due to glycosylation.

实验方案

Product Specific Protocols
WB protocol for TSPAN9 antibody 21983-1-APDownload protocol
IP protocol for TSPAN9 antibody 21983-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouse,ratWB,FC

Nat Commun

Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model

Authors - Mengyan Hu
humanWB

Adv Sci (Weinh)

Bone Marrow Mesenchymal Stem Cell-Derived Dermcidin-Containing Migrasomes enhance LC3-Associated Phagocytosis of Pulmonary Macrophages and Protect against Post-Stroke Pneumonia

Authors - Tiemei Li