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验证数据展示

  • WB analysis using 20319-1-AP (same clone as 20319-1-PBS)

    WB analysis using 20319-1-AP (same clone as 20319-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20319-1-AP (TSPAN8 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20319-1-PBS in a different storage buffer formulation.

  • IP experiment of PC-12 using 20319-1-AP (same clone as 20319-1-PBS)

    IP experiment of PC-12 using 20319-1-AP (same clone as 20319-1-PBS)

    IP result of anti-TSPAN8 (IP:20319-1-AP, 4ug; Detection:20319-1-AP 1:1000) with PC-12 cells lysate 1560 ug. This data was developed using the same antibody clone with 20319-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 20319-1-AP (same clone as 20319-1-PBS)

    IHC staining of human stomach cancer using 20319-1-AP (same clone as 20319-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 20319-1-AP (TSPAN8 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20319-1-PBS in a different storage buffer formulation.

TSPAN8 Polyclonal antibody, PBS Only

TSPAN8 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 20319-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IHC, IP, Indirect ELISA

别名

CO 029, tetraspanin 8, Tetraspanin-8, TM4SF3, Tspan 8

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

20319-1-PBS targets TSPAN8 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, rat
免疫原

CatNo: Ag14173

Product name: Recombinant human TSPAN8 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 97-217 aa of BC005246

Sequence: LLQVATGILGAVFKSKSDRIVNETLYENTKLLSATGESEKQFQEAIIVFQEEFKCCGLVNGAADWGNNFQHYPELCACLDKQRPCQSYNGKQVYKETCISFIKDFLAKNLIIVIGIAFGLA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tetraspanin 8
别名 CO 029, tetraspanin 8, Tetraspanin-8, TM4SF3, Tspan 8
计算分子量 237 aa, 26 kDa
观测分子量27 kDa
GenBank蛋白编号BC005246
基因名称 TSPAN8
Gene ID (NCBI) 7103
RRIDAB_3741986
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP19075
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TSPAN8 is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Tetraspanins have been involved in diverse processes such as cell activation and proliferation, adhesion and motility, differentiation, and cancer. TSPAN8 and CD151 coordinately promote metastasis, where Tspan8 overrides the adhesive features of CD151 by recruiting integrins out of adhesion into motility promoting complexes (PMID: 23683890). TSPAN8 contributes to molecular pathways of exosome-induced endothelial cell activation (PMID: 20124479).

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