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TSPAN5 Polyclonal antibody

TSPAN5 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat No : 12122-1-AP

Print datasheet

Synonyms

NET 4, NET4, Tetraspan NET 4, tetraspanin 5, TM4SF9, TSPAN 5, TSPAN5

验证数据展示

  • WB analysis of Jurkat using 12122-1-AP

    WB analysis of Jurkat using 12122-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 12122-1-AP (TSPAN5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of Jurkat using 12122-1-AP

    WB analysis of Jurkat using 12122-1-AP

    WB result of TSPAN5 antibody (12122-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TSPAN5 transfected Jurkat cells.

经过测试的应用

Positive WB detected inJurkat cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

12122-1-AP targets TSPAN5 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen TSPAN5 fusion protein Ag2765 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tetraspanin 5
Synonyms NET 4, NET4, Tetraspan NET 4, tetraspanin 5, TM4SF9, TSPAN 5, TSPAN5
Calculated Molecular Weight 268 aa, 30 kDa
Observed Molecular Weight 30-40 kDa, 90 kDa
GenBank Accession NumberBC009704
Gene Symbol TSPAN5
Gene ID (NCBI) 10098
RRIDAB_10638611
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP62079
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TSPAN5 is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Tetraspanins have been involved in diverse processes such as cell activation and proliferation, adhesion and motility, differentiation, and cancer. TSPAN5 belongs to the TSPANC8 subfamily, which also include TSPAN10, TSPAN14, TSPAN15, TSPAN17, and TSPAN33. It has been reported that TSPANC8 tetraspanins interact with ADAM10 and have a conserved function in the regulation of ADAM10 trafficking and activity, thereby positively regulating Notch receptor activation (PMID: 23091066; 23035126).

实验方案

Product Specific Protocols
WB protocol for TSPAN5 antibody 12122-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Commun

Phenylalanine-tRNA aminoacylation is compromised by ALS/FTD-associated C9orf72 C4G2 repeat RNA

Authors - Mirjana Malnar Črnigoj
View Article
humanWB

Heliyon

Susceptibility of TSPAN5 polymorphisms to Kawasaki disease and immunoglobulin therapy resistance in southern Chinese children

Authors - Kaining Chen
View Article